IGF1R Human ELISA Kit/人IGF1R ELISA试剂盒
货号：EHIGF1R,EHIGF1RX5,EHIGF1RX10
规格：96 tests,5X96 tests,10X96 tests
价格：7238,29826,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 6 pg/mL |
| 检测范围 | 8.23-6000 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 50 μL 血浆 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | IGF1R (CD221) |
| Protein aliases | CD221, IGF-I receptor, Insulin-like growth factor 1 receptor, Insulin-like growth factor 1 receptor alpha chain, Insulin-like growth factor 1 receptor beta chain, Insulin-like growth factor I receptor, soluble IGF1R variant 1, soluble IGF1R variant 2 |
物种（Tested） | 人 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | CD221, IGF1R, IGFIR, IGFR, JTK13 |
| Gene ID | (Human) 3480 |
| Gene symbol | IGF1R |
| UniProt ID | (Human) P08069 |
| The Human Insulin-like Growth Factor 1R (Hu IGF1R) ELISA quantitates Hu IGF1R in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IGF1R.人胰岛素样生长因子1R（人IGF1R）ELISA定量了人血清，血浆或细胞培养基中的人IGF1R。 该检测试剂盒将专门识别天然和重组的人IGF1R。Principle of the method 方法原理The Human IGF1R solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人IGF1R固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Insulin growth factor 1 receptor belongs to the family of tyrosine receptor kinases. It is involved in activation of various substrates including IGF and neurotensin, regulation of metabolism, cell division, maintenance, and inflammation response. The protein is a tetramer consisting of two alpha, and two beta subunits. The alpha-subunits binds to insulin growth factor 1 with higher affinity than to insulin growth factor 2. Binding results in a conformational change, followed by autophosphorylation of IGF-1R at positions 1134, 1135, 1136. The activated IGF-1R in turn, causes phosphorylation of substrate proteins, followed by sequential activation of RAS, RAF, and mitogen-activated protein kinase isoforms ERK, p38, and JNK, leading to the transcription of genes that drive proliferation. This antibody recognizes the beta-subunit of IGF-1R.胰岛素生长因子受体1属于酪氨酸受体激酶家族。 它涉及各种底物的激活，包括IGF和神经降压素，代谢的调节，细胞分裂，维持和炎症反应。 该蛋白质是由两个α和两个β亚基组成的四聚体。 α亚基以比胰岛素生长因子2高的亲和力与胰岛素生长因子1结合。结合导致构象变化，随后IGF-1R在位置1134、1135、1136发生自磷酸化。激活的IGF-1R依次是 引起底物蛋白的磷酸化，随后依次激活RAS，RAF和丝裂原激活的蛋白激酶同工型ERK，p38和JNK，从而导致驱动增殖的基因转录。 该抗体识别IGF-1R的β亚基。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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