TWEAK (TNFSF12) Human ELISA Kit/人TWEAK (TNFSF12) ELISA试剂盒
货号：EHTNFSF12,EHTNFSF12X5,EHTNFSF12X10
规格：96 tests,5X96 tests,10X96 tests
价格：7238,29826,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 40 pg/mL |
| 检测范围 | 46.88-3000 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 33 μL 血浆 33 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | TWEAK |
| Protein aliases | APO3 ligand, TNF-related weak inducer of apoptosis, TWEAK, APO3/DR3 ligand, TNF-related WEAK inducer of apoptosis, tumor necrosis factor (ligand) superfamily member 12, tumor necrosis factor (ligand) superfamily, member 12, tumor necrosis factor ligand 4A, Tumor necrosis factor ligand superfamily member 12, membrane form, secreted form |
物种（Tested） | 人 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | APO3L, DR3LG, TNFSF12, TNLG4A, TWEAK, UNQ181/PRO207 |
| Gene ID | (Human) 8742 |
| Gene symbol | TNFSF12 |
| UniProt ID | (Human) O43508 |
| The Human TWEAK (TNFSF12) ELISA quantitates Hu TWEAK in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TWEAK.人TWEAK（TNFSF12）ELISA定量了人血清，血浆或细胞培养基中的人TWEAK。 该检测试剂盒将专门识别天然和重组的人TWEAK。Principle of the method 方法原理The Human TWEAK solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人TWEAK固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息TNF-related weak inducer of apoptosis (TWEAK) is a member of the tumor necrosis factor superfamily (TNFSF) of structurally related cytokines. Like most other members of this family, TWEAK is a cell surface-associated type II transmembrane protein although a smaller, biologically active form can also be generated by cleavage near the cell membrane. TWEAK has multiple biological activities, including stimulation of cell growth and angiogenesis, induction of inflammatory cytokines, in addition to stimulation of apoptosis. The TWEAK signal transduction pathway has not been well established but it appears to signal via TweakR/Fn14 in a manner similar to that described for other TNFSF members that bind receptors lacking death domains.TNF相关的凋亡弱诱导剂（TWEAK）是肿瘤坏死因子超家族（TNFSF）的成员。 像该家族的大多数其他成员一样，TWEAK是一种细胞表面相关的II型跨膜蛋白，尽管较小的生物活性形式也可以通过在细胞膜附近裂解而产生。 TWEAK具有多种生物学活性，除了刺激细胞凋亡外，还包括刺激细胞生长和血管生成，诱导炎性细胞因子。 TWEAK信号转导途径尚未很好地建立，与针对其他结合缺乏死亡域的受体的TNFSF成员通过TweakR / Fn14发出信号。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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