SAA Human ELISA Kit/人SAA ELISA试剂盒
货号：EHSAA1,EHSAA1X5,EHSAA1X10
规格：96 tests,5X96 tests,10X96 tests
价格：7311,30065,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 500 pg/mL |
| 检测范围 | 0.41-300 ng/mL |
样本类型/体积 | 上清液 100 μL 血清 50 μL 血浆 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | SAA |
| Protein aliases | Amyloid fibril protein AA, Amyloid protein A, SAA, serum amyloid A protein, Serum amyloid A-1 protein, Serum amyloid protein A(2-102), Serum amyloid protein A(2-103), Serum amyloid protein A(2-104), Serum amyloid protein A(3-104), Serum amyloid protein A(4-101), tumor protein p53 inducible protein 4 |
物种（Tested） | 人 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | PIG4, SAA, SAA1, SAA2, TP53I4 |
| Gene ID | (Human) 6288 |
| Gene symbol | SAA1 |
| UniProt ID | (Human) P0DJI8 |
| The Human Serum Amyloid A (Hu SAA) ELISA quantitates Hu SAA in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu SAA.人血清淀粉样蛋白A（人SAA）ELISA定量了人血清，血浆或细胞培养基中的人SAA。 该检测试剂盒将专门识别天然和重组人SAA。Principle of the method 方法原理The Human SAA solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人SAA固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Serum amyloid A (SAA) proteins comprise a family of small (12-14 kDa, 104-112 amino acid residues), differentially expressed proteins that are highly conserved among vertebrates. SAA proteins are involved in the acute phase responses; these are the immediate early host responses to inflammation. During the acute phase, circulating SAA levels are increased by 100-1000 fold, reaching concentrations of up to one milligram per milliliter. Liver is the major site of SAA synthesis, although extrahepatic expression has also been reported. Mouse SAA gene family maps to chromosome 7. In mouse, five SAA genes and four protein products have been identified: mouse SAA1, SAA2, SAA3 are the acute phase isoforms, SAA5 is constitutively expressed, and SAA4 is a pseudogene. SAAs belong to a category of acute phase proteins that also includes C-reactive protein (CRP), haptoglobin, ceruloplasmin, complement components C3 and C4, a1-acid glycoprotein, a1-proteinase inhibitor, and fibrinogen.血清淀粉样蛋白A（SAA）蛋白包含一族小的（12-14 kDa，104-112个氨基酸残基）差异表达的蛋白，这些蛋白在脊椎动物中高度保守。 SAA蛋白参与急性期反应。 这些是宿主对炎症的早期反应。 在急性期，循环SAA水平增加了100-1000倍，达到最高浓度为每毫升1毫克。 肝脏是SAA合成的主要部位，尽管也有肝外表达的报道。 小鼠SAA基因家族定位于7号染色体。在小鼠中，已鉴定出5个SAA基因和4种蛋白质产物：小鼠SAA1，SAA2，SAA3为急性期同工型，组成性表达SAA5，而SAA4为假基因。 SAA属于一类急性期蛋白，还包括C反应蛋白（CRP），触珠蛋白，铜蓝蛋白，补体成分C3和C4，α1-酸糖蛋白，α1-蛋白酶抑制剂和纤维蛋白原。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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