CHI3L1/YKL-40 Human ELISA Kit/人CHI3L1/YKL-40 ELISA试剂盒
货号：EHCHI3L1,EHCHI3L1X5,EHCHI3L1X10
规格：96 tests,5X96 tests,10X96 tests
价格：7311,29891,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 6 pg/mL |
| 检测范围 | 6.14-1500 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 1 μL 血浆 1 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | CHI3L1 |
| Protein aliases | 39 kDa synovial protein, Cartilage glycoprotein 39, CGP-39, GP-39, hCGP-39, YKL-40, chitinase 3-like 1 (cartilage glycoprotein-39), Chitinase-3-like protein 1 |
物种（Tested） | 人 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | ASRT7, CGP-39, CHI3L1, GP-39, GP39, HC-gp39, hCGP-39, HCGP-3P, YKL-40, YKL40, YYL-40 |
| Gene ID | (Human) 1116 |
| Gene symbol | CHI3L1 |
| UniProt ID | (Human) P36222 |
| The Human CHI3L1/YKL-40 ELISA quantitates Hu CHI3L1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu CHI3L1.人CHI3L1 / YKL-40 ELISA定量检测人血清，血浆或细胞培养基中的Hu CHI3L1。 该检测试剂盒将只识别天然和重组的Hu CHI3L1。Principle of the method 方法原理The Human CHI3L1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人CHI3L1固相夹心ELISA（酶联免疫吸附测定）是测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Human cartilage glycoprotein 39 (GP-39), also known as YKL-40, is a glycoprotein secreted by articular chondrocytes, synoviocytes and macrophages. Serum and synovial fluid GP-39 levels are elevated in inflammatory diseases and correlate with the degree of joint destruction in rheumatoid arthritis. GP-39 is expressed in articular chondrocytes and synovial cells, as well as in liver, but is undetectable in muscle tissues, lung, pancreas, mononuclear cells and fibroblasts. GP-39 is a candidate autoantigen in rheumatoid arthritis and is important in the capacity of cells to respond to and cope with changes in their environment.人软骨糖蛋白39（GP-39），也称为YKL-40，是关节软骨细胞，滑膜细胞和巨噬细胞分泌的糖蛋白。 血清和滑液GP-39水平在炎性疾病中升高，并与类风湿关节炎的关节破坏程度相关。 GP-39在关节软骨细胞和滑膜细胞以及肝脏中表达，但在肌肉组织，肺，胰腺，单核细胞和成纤维细胞中无法检测到。 GP-39是类风湿关节炎的候选自身抗原，对于细胞应对和应对环境变化的能力至关重要。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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