FGF-7 (KGF) Human ELISA Kit/人FGF-7 (KGF) ELISA试剂盒

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FGF-7 (KGF) Human ELISA Kit/人FGF-7 (KGF) ELISA试剂盒

货号:EHFGF7,EHFGF7X5,EHFGF7X10

规格:96 tests,5X96 tests,10X96 tests

价格:7238,29891,52892

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

0.5 pg/mL

检测范围0.55-400 pg/mL

样本类型/体积

上清液 100 μL

血清 50 μL

血浆 50 μL

操作时间

1 hr 20 min

测试时间

4 hr 45 min

Homogenous (no wash)

No

批间变异系数(CV)<12%
批内变异系数(CV)<10%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

96 Tests

组份

- 预包被96孔板

- 标准品

- 分析稀释浓缩液

- 生物素化检测抗体

-SAV-HRP

- 洗涤液

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameKGF
Protein aliasesFGF-7, Heparin-binding growth factor 7, HBGF-7, Keratinocyte growth factor, Fibroblast growth factor 7

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesFGF7, HBGF-7, KGF
Gene ID(Human) 2252
Gene symbolFGF7
UniProt ID

(Human) P21781

The Human Fibroblast Growth Factor 7/KGF (Hu FGF7) ELISA quantitates Hu FGF7 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu FGF7.人成纤维细胞生长因子7 / KGF(Hu FGF7)ELISA定量了人血清,血浆或细胞培养基中的人FGF7。 该检测试剂盒将只识别天然和重组人FGF7。Principle of the method 方法原理The Human FGF7 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人FGF7固相夹心ELISA(酶联免疫吸附测定)设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis.该基因编码的蛋白质是成纤维细胞生长因子(FGF)家族的成员。 FGF家族成员具有广泛的促有丝分裂和细胞存活活性,并参与多种生物学过程,包括胚胎发育,细胞生长,形态发生,组织修复,肿瘤生长和侵袭。 该蛋白是有效的上皮细胞特异性生长因子,其促有丝分裂活性主要表现在角质形成细胞中,而不表现在成纤维细胞和内皮细胞中。 对该基因的小鼠和大鼠同源物的研究涉及上皮的形态发生,伤口的再上皮化,头发发育和早期肺器官发生中的作用。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
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