IL-10 Canine ELISA Kit/犬类IL-10 ELISA试剂盒
货号：ECIL10,ECIL10X5,ECIL10X10
规格：96 tests,5X96 tests,10X96 tests
价格：7238,29891,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：其它
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 0.1 ng/mL |
| 检测范围 | 0.11-80 ng/mL |
样本类型/体积 | 上清液 100 μL 血清 50 μL 血浆 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | IL-10 |
| Protein aliases | CSIF, Cytokine synthesis inhibitory factor, IL-10, Interleukin-10 |
物种（Tested） | 狗 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | IL-10, IL10 |
| Gene ID | (Dog) 403628 |
| Gene symbol | IL10 |
| UniProt ID | (Dog) P48411 |
| The Canine Interleukin 10 (Dog IL-10) ELISA quantitates Dog IL-10 in canine serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Dog IL-10.犬白介素10（狗IL-10）ELISA定量犬血清，血浆或细胞培养基中的狗IL-10。 该检测试剂盒将专门识别天然和重组狗IL-10。Principle of the method 方法原理The Canine IL-10 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.犬IL-10固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Interleukin-10 is a pleiotropic cytokine playing an important role as a regulator of lymphoid and myeloid cell function. In humans IL-10 is produced by activated CD8(+) peripheral blood T-cells, by T-helper CD4(+) T-cell clones. Due to the ability of IL-10 to block cytokine synthesis and several accessory cell functions of macrophages this cytokine is a potent suppressor of the effector functions of macrophages, T-cells and NK cells. In addition, IL-10 participates in regulating proliferation and differentiation of B-cells, mast cells and thymocytes. The primary structure of human IL-10 has been determined by cloning the cDNA encoding the cytokine. The corresponding protein exerts 160 amino acids with a predicted molecular mass of 18.5 kDa. Based on its primary structure, IL-10 is a member of the four a-helix bundle family of cytokines. In solution human IL-10 is a homodimer with an apparent molecular mass of 39 kDa. Although it contains an N-linked, glycosylation site, it lacks detectable carbohydrates. Recombinant protein expressed in E. coli thus retains all known biological activities. The human IL-10 gene is located on chromosome 1 and is present as a single copy in the genome. The human IL-10 exhibits strong DNA and amino acid sequence homology to the murine IL-10 and an open reading frame in the Epstein-Barr virus genome, BCRF1 which shares many of the cellular cytokine's biological activities and may therefore play a role in the host-virus interaction. The immunosuppressive properties of IL-10 suggest a possible clinical use of IL-10 in suppressing rejections of grafts after organ transplantations. IL-10 can furthermore exert strong anti-inflammatory activities.白介素10是一种多效细胞因子，在调节淋巴样和髓样细胞功能中起着重要作用。在人类中，IL-10由活化的CD8（+）外周血T细胞，T辅助CD4（+）T细胞克隆产生。由于IL-10具有阻断细胞因子合成的能力和巨噬细胞的几种辅助细胞功能，因此该细胞因子是巨噬细胞，T细胞和NK细胞的效应子功能的有效抑制剂。另外，IL-10参与调节B细胞，肥大细胞和胸腺细胞的增殖和分化。已经通过克隆编码细胞因子的cDNA来确定人IL-10的一级结构。相应的蛋白质具有160个氨基酸，预测分子量为18.5 kDa。根据其主要结构，IL-10是细胞因子的四个a-螺旋束家族的成员。在溶液中，人IL-10是表观分子量为39kDa的同型二聚体。尽管它包含一个N连接的糖基化位点，但它缺乏可检测的碳水化合物。因此，在大肠杆菌中表达的重组蛋白保留了所有已知的生物学活性。人IL-10基因位于1号染色体上，在基因组中以单拷贝形式存在。人IL-10与鼠类IL-10具有很强的DNA和氨基酸序列同源性，并在爱泼斯坦-巴尔病毒基因组BCRF1中具有开放阅读框，BCRF1具有许多细胞因子的生物学活性，因此可能在其中发挥作用。主机与病毒的交互。 IL-10的免疫抑制特性表明IL-10在抑制器官移植后移植物排斥反应方面的临床应用可能。 IL-10还可以发挥强大的抗炎活性。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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