BCAM Human ELISA Kit/人BCAM ELISA试剂盒
货号：EHBCAM,EHBCAMX5,EHBCAMX10
规格：96 tests,5X96 tests,10X96 tests
价格：7238,29826,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 40 pg/mL |
| 检测范围 | 40.96-10,000 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 33 μL 血浆 33 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | BCAM |
| Protein aliases | antigen identified by monoclonal antibody F8, Auberger B antigen, B-CAM cell surface glycoprotein, B-cell adhesion molecule, Basal cell adhesion molecule, basal cell adhesion molecule (Lu and Au blood groups), basal cell adhesion molecule Lutheran blood group, CD239, F8/G253 antigen, glycoprotein 95kDa, Lutheran antigen, Lutheran blood group, Lutheran blood group glycoprotein, Lutheran blood group variant LUGA |
物种（Tested） | 人 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | AU, BCAM, CD239, LU, MSK19 |
| Gene ID | (Human) 4059 |
| Gene symbol | BCAM |
| UniProt ID | (Human) P50895 |
| The Human Basal Cell Adhesion Molecule (Hu BCAM) ELISA quantitates Hu BCAM in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu BCAM.人基底细胞粘附分子（Hu BCAM）ELISA可以定量人血清，血浆或细胞培养基中的Hu BCAM。 该检测试剂盒将专门识别天然和重组Hu BCAM。Principle of the method 方法原理The Human BCAM solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人BCAM固相夹心ELISA（酶联免疫吸附测定）是测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息BDNF (Brain-Derived Neurotrophic Factor) is a member of the neurotrophin family. BDNF is synthesized as pre-proBDNF, followed by cleavage to proBDNF. Although further processing generates the mature, 14 kDa protein, pro-BDNF is biologically active and is secreted in synaptic vesicles along with the mature form. BDNF is widely expressed in the central nervous system and acts in an autocrine and paracrine manner on several classes of neurons. Signaling occurs mainly through the tyrosine kinase receptor TrkB, although binding to the lower-affinity receptor, p75-NTR, has also been demonstrated. BDNF promotes neuronal survival and differentiation and has been shown to play a critical role in memory formation and synaptic regulation. BDNF is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of BDNF is reduced in both Alzheimer's and Huntington disease patients, and may also play a role in the regulation of stress response and in the biology of mood disorders.BDNF（脑源性神经营养因子）是Neurotrophin家族的成员。 BDNF合成为pre-proBDNF，然后裂解为proBDNF。尽管进一步加工产生了成熟的14 kDa蛋白，但pro-BDNF具有生物学活性，并与成熟形式一起分泌在突触小泡中。 BDNF在中枢神经系统中广泛表达，并以自分泌和旁分泌方式作用于几种神经元。信号主要通过酪氨酸激酶受体TrkB发生，尽管也已证明与低亲和力受体p75-NTR结合。 BDNF促进神经元的存活和分化，并已被证明在记忆形成和突触调节中起关键作用。 BDNF由皮层神经元诱导，对于大脑中纹状体神经元的生存是必需的。 BDNF的表达在阿尔茨海默氏病和亨廷顿病患者中均降低，并且也可能在应激反应的调节和情绪障碍的生物学中起作用。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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