G-CSF (CSF3) Mouse ELISA Kit/小鼠G-CSF (CSF3) ELISA试剂盒
货号：EMCSF3,EMCSF3X5,EMCSF3X10
规格：96 tests,5X96 tests,10X96 tests
价格：7311,29891,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：小鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 0.5 pg/mL |
| 检测范围 | 0.62-150 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 5 μL 血浆 5 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | G-CSF |
| Protein aliases | G-CSF, Granulocyte colony-stimulating factor |
物种（Tested） | 小鼠 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | Csf3, Csfg, G-CSF, MGI-IG |
| Gene ID | (Mouse) 12985 |
| Gene symbol | Csf3 |
| UniProt ID | (Mouse) P09920 |
| The Mouse G-CSF (CSF3) ELISA quantitates Ms G-CSF in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms G-CSF.小鼠G-CSF（CSF3）ELISA定量检测小鼠血清，血浆或细胞培养基中的Ms G-CSF。 该检测试剂盒将专门识别天然和重组Ms G-CSF。Principle of the method 方法原理The Mouse G-CSF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.小鼠G-CSF固相夹心ELISA（酶联免疫吸附测定）是测量匹配的抗体对之间结合的靶标量。 已在孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息G-CSF (Granulocyte colony stimulating factor) is a naturally occurring cytokine that stimulates the production and antibacterial function of neutrophils and monocytes. Human G-CSF is an 18.8 kDa protein containing 175 amino acid residues, and a soluble isoform of the G-CSF receptor has been described. The pleotropic cytokine is produced by activated monocytes, macrophages, endothelial cells, fibroblasts, astrocytes, osteoblasts and bone marrow cells. G-CSF has been shown to have specific effects on the proliferation, differentiation and activation of hematopoietic cells. G-CSF is also expressed by various transformed cells such as carcinoma cells and myeloblastic leukemia cells. G-CSF is encoded by two distinct DNA sequences, resulting in a full size, high activity and a shorter, low activity isoform of G-CSF. G-CSF is highly conserved among species and has been shown to exert its biological functions through interaction with its receptor expressed on the surface of hematopoietic progenitors, neutrophilic granulocytes and certain carcinoma cell lines. Clinical use of G-CSF has been approved for several therapeutic applications, treatment of neonatal infections, therapy of acute myocardial infarction, granulocyte transfusion in patients with neutropenia, in severe infections and sepsis, therapy in chronic autoimmune neutropenia, treatment of acute myeloid leukemias, Sweet's syndrome and AIDS. Further, G-CSF has been shown to be a marker protein for different carcinomas such as bladder cancer and dysfunction of the protein has been linked to Kostmann Syndrome.G-CSF（粒细胞集落刺激因子）是一种天然存在的细胞因子，可刺激嗜中性粒细胞和单核细胞的产生和抗菌功能。人G-CSF是一种含有175个氨基酸残基的18.8kDa蛋白质，并且已经描述了G-CSF受体的可溶性同工型。多效性细胞因子由活化的单核细胞，巨噬细胞，内皮细胞，成纤维细胞，星形胶质细胞，成骨细胞和骨髓细胞产生。已经显示G-CSF对造血细胞的增殖，分化和活化具有特定作用。 G-CSF还可以通过多种转化细胞表达，例如癌细胞和粒细胞白血病细胞。 G-CSF由两个不同的DNA序列编码，从而导致G-CSF的完整大小，高活性和较短，低活性的亚型。 G-CSF在物种之间是高度保守的，并且已显示出通过与其在造血祖细胞，嗜中性粒细胞和某些癌细胞系表面表达的受体的相互作用而发挥其生物学功能。 G-CSF的临床应用已获批准用于多种治疗应用，新生儿感染的治疗，中性粒细胞减少症患者的粒细胞输血，严重感染和败血症的治疗，慢性自身免疫性中性粒细胞减少症的治疗，急性髓细胞性白血病的治疗，甜氏综合症和艾滋病。此外，已经表明G-CSF是用于不同癌症例如膀胱癌的标志物蛋白，并且该蛋白的功能障碍与Kostmann综合症有关。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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