PGFM Enzyme Competitive ELISA Kit/PGFM酶竞争性ELISA试剂盒

货号：EIAPGFM,EIAPGFMX10

规格：96 tests,10X96 tests

价格：7238,52892

产品类型：ELISA检测

品牌：Thermo Fisher

物种：其它

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	20.8 pg/mL
检测范围	50-3200 pg/mL
体积	粪便提取物 50 µL 血浆 6.25 µL 血清 6.25 µL 上清液 50 µL 尿液 6.25 µL
操作时间	1 hr 20 min
测试时间	1 hr 30 min
Homogenous (no wash)	No
批间变异系数（CV）	9.7%
批内变异系数（CV）	9.2%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	96 Tests
组份	- 预包被96孔板 - 标准品 - 测定稀释液 - HRP-共轭检测抗体 - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	-20°C
Protein name	PGFM
Protein aliases	C20H34O5, 13, 14-dihydro-15-keto-PGF2alpha, 14-dihydro-15-keto PGF2a, 14-dihydro-15-keto PGF2 alpha
物种（Tested）	Chemical
试剂盒类型	Competitive ELISA
标记（染料）	HRP
检测抗体偶联物	HRP

The PGFM Enzyme ELISA quantitates PGFM in serum, plasma, urine, dried fecal extracts, or cell culture medium.PGFM酶ELISA定量分析血清，血浆，尿液，干燥的粪便提取物或细胞培养基中的PGFM。Principle of the method 方法原理The PGFM Competitive ELISA research-use-only kit designed to quantitatively measure PGFM present in fecal extracts, urine, serum, and plasma samples. A PGFM standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit IgG. A PGFM-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a rabbit polyclonal antibody highly specific to PGFM to each well. After a 1-hour incubation, the plate is washed and substrate is added. The substrate reacts with the bound PGFM-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.PGFM竞争性ELISA试剂盒，用于定量测量粪便提取物，尿液，血清和血浆样品中的PGFM。 提供了PGFM标准品以生成测定的标准曲线，并且应从标准曲线中读取所有样品。 将标准品或稀释的样品吸移到涂有抗体的透明微量滴定板中，以捕获兔IgG。 将PGFM-过氧化物酶结合物添加到孔中的标准品和样品中。 通过向每个孔中添加对PGFM具有高度特异性的兔多克隆抗体来启动结合反应。 温育1小时后，洗涤板并加入底物。 底物与结合的PGFM-过氧化物酶偶联物反应。 短暂孵育后，终止反应，并在能够在450 nm处测量的微量滴定板读数器中检测所产生颜色的强度。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息In many species, uterine and placental Prostaglandin F2alpha (PGFM) is involved in the regulation of reproductive and pregnancy-related processes such as embryonic development, initiation of parturition, and resumption of ovarian activity. Prostaglandin F2alpha is metabolized to PGFM during the first passage through the lungs. PGFM has a longer half-life in peripheral circulation than PGF2alpha and has been applied as a useful analytical marker of PGF2alpha. PGFM is identical across all species and so this kit should measure PGFM from sources other than those tested.在许多物种中，子宫和胎盘前列腺素F2alpha（PGFM）参与生殖和妊娠相关过程的调控，例如胚胎发育，分娩开始和卵巢活性恢复。 前列腺素F2alpha在第一次通过肺部时被代谢为PGFM。 PGFM在外周循环中的半衰期比PGF2alpha更长，已被用作PGF2alpha的有用分析标记。 PGFM在所有物种中都是相同的，因此该试剂盒应从非测试来源测量PGFM。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。

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