FGF4 Human ELISA Kit/人FGF4 ELISA试剂盒

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FGF4 Human ELISA Kit/人FGF4 ELISA试剂盒

货号:EHFGF4,EHFGF4X5,EHFGF4X10

规格:96 tests,5X96 tests,10X96 tests

价格:6896,28416,50393

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

50 pg/mL

检测范围24.69-18,000 pg/mL

样本类型/体积

上清液 100 μL

血清 50 μL

血浆 50 μL

操作时间

1 hr 20 min

测试时间

4 hr 45 min

Homogenous (no wash)

No

批间变异系数(CV)<12%
批内变异系数(CV)<10%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

96 Tests

组份

- 预包被96孔板

- 标准品

- 分析稀释浓缩液

- 生物素化检测抗体

-SAV-HRP

- 洗涤液

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameFGF4
Protein aliasesFGF-4, Fibroblast growth factor 4, fibroblast growth factor 4 splice isoform, HBGF-4, heparin secretory transforming protein 1, Heparin secretory-transforming protein 1, Heparin-binding growth factor 4, HST, HSTF-1, human stomach cancer, transforming factor from FGF-related oncogene, kaposi sarcoma oncogene, oncogene HST, Transforming protein KS3

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesFGF4, HBGF-4, HST, HST-1, HSTF1, K-FGF, KFGF, KS3
Gene ID(Human) 2249
Gene symbolFGF4
UniProt ID

(Human) P08620

The Human Fibroblast Growth Factor 4 (Hu FGF4) ELISA quantitates Hu FGF4 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu FGF4.人成纤维细胞生长因子4(Hu FGF4)ELISA定量了人血清,血浆或细胞培养基中的人FGF4。 该检测试剂盒将只识别天然和重组的人FGF4。Principle of the method 方法原理The Human FGF4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人FGF4固相夹心ELISA(酶联免疫吸附测定)设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息Fibroblast growth factor 4 (FGF4) is a member of the fibroblast growth factor (FGF) family that possess broad mitogenic and cell survival activities and play key roles in growth and survival of stem cells during embryogenesis, tissue regeneration, and carcinogenesis. FGF4 was identified by its strong oncogenic transforming activity and is a potent angiogenic factor, expressed in several highly vascularized tumors and also in adult mouse testis, intestine, and brain. Studies on the mouse homolog suggests a function in bone morphogenesis and limb development through the sonic hedgehog (SHH) signaling pathway. Furthermore, FGF4 regulates neural progenitor cell proliferation and neuronal differentiation. Recent studies show a growth-promoting role for FGF4 in human embryonic stem cells and a putative feedback inhibition mechanism by a novel FGF4 splice isoform that may serve to promote differentiation at a later stages of development.成纤维细胞生长因子4(FGF4)是成纤维细胞生长因子(FGF)家族的成员,具有广泛的促有丝分裂和细胞存活活性,并且在胚胎发生,组织再生和癌变过程中在干细胞的生长和存活中起关键作用。 FGF4通过其强大的致癌转化活性而被鉴定,并且是有效的血管生成因子,在几种高度血管化的肿瘤以及成年小鼠睾丸,肠和脑中均有表达。 对小鼠同源物的研究表明,通过声波刺猬(SHH)信号传导途径在骨形态发生和四肢发育中起作用。 此外,FGF4调节神经祖细胞增殖和神经元分化。 最近的研究表明,FGF4在人类胚胎干细胞中具有促进生长的作用,并且通过新型FGF4剪接同工型推定的抑制反馈机制,可在以后的发育阶段促进分化。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
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