IL-36G (IL-1F9) Human ELISA Kit/人IL-36G (IL-1F9) ELISA试剂盒
货号：EHIL36G,EHIL36GX5,EHIL36GX10
规格：96 tests,5X96 tests,10X96 tests
价格：7238,29891,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 13 pg/mL |
| 检测范围 | 12.29-3000 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 50 μL 血浆 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | IL1F9 |
| Protein aliases | IL-1 epsilon, IL-1 related protein 2, IL-1(EPSILON), IL-1-epsilon, IL-1-related protein 2, IL-1F9, IL-1H1, IL-1RP2, interleukin 1 family, member 9, interleukin 1-related protein 2, Interleukin-1 epsilon, Interleukin-1 family member 9, Interleukin-1 homolog 1, Interleukin-36 gamma |
物种（Tested） | 人 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | IL-1F9, IL-1H1, IL-1RP2, IL1E, IL1F9, IL1H1, IL1RP2, IL36G, UNQ2456/PRO5737 |
| Gene ID | (Human) 56300 |
| Gene symbol | IL36G |
| UniProt ID | (Human) Q9NZH8 |
| The Human Interleukin 36G (IL-36G/IL-1F9) ELISA quantitates Hu IL-36G in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-36G.人白介素36G（IL-36G / IL-1F9）ELISA定量了人血清，血浆或细胞培养基中的人IL-36G。 该经检测试剂盒将只识别天然和重组的人IL-36G。Principle of the method 方法原理The Human IL-36G solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人IL-36G固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. In cultured keratinocytes induces the expression of macrophage, T-cell, and neutrophil chemokines, such as CCL3, CCL4, CCL5, CCL2, CCL17, CCL22, CL20, CCL5, CCL2, CCL17, CCL22, CXCL8, CCL20 and CXCL1; also stimulates its own expression and that of the prototypic cutaneous proinflammatory parameters TNF-alpha, S100A7/psoriasin and inducible NOS. May play a role in proinflammatory responses during particular neutrophilic airway inflammation: activates mitogen-activated protein kinases and NF-kappa B in primary lung fibroblasts, and stimulates the expression of IL-8 and CXCL3 and Th17 chemokine CCL20 in lung fibroblasts. May be involved in the innate immune response to fungal pathogens, such as Aspergillus fumigatus.与IL1RL2 / IL-36R受体结合并通过其发出信号的细胞因子，进而激活靶细胞中的NF-κB和MAPK信号通路。 IL-36是信号传导系统的一部分，被认为存在于上皮屏障中，并参与局部炎症反应；类似于与IL-1系统共享共受体IL1RAP的系统。似乎通过作用于角质形成细胞，树突状细胞以及间接作用于T细胞以驱动组织浸润，细胞成熟和细胞增殖而参与皮肤炎症反应。在培养的角质形成细胞中诱导巨噬细胞，T细胞和嗜中性粒细胞趋化因子的表达，例如CCL3，CCL4，CCL5，CCL2，CCL17，CCL22，CL20，CCL5，CCL2，CCL17，CCL22，CXCL8，CCL20和CXCL1的表达;还刺激其自身表达以及原型皮肤促炎参数TNF-α，S100A7 / psoriasin和诱导型NOS的表达。在特定的嗜中性气道炎症过程中可能在促炎反应中起作用：激活初级肺成纤维细胞中的促分裂原活化蛋白激酶和NF-κB，并刺激肺成纤维细胞中IL-8和CXCL3和Th17趋化因子CCL20的表达。可能参与了对真菌病原体（如烟曲霉）的先天免疫应答。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-20578.htm
QQ:1749072012