TIMP2 Human ELISA Kit/人TIMP2 ELISA试剂盒
货号：EHTIMP2,EHTIMP2X5,EHTIMP2X10
规格：96 tests,5X96 tests,10X96 tests
价格：7238,29891,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 2 pg/mL |
| 检测范围 | 1.6-1200 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 1 μL 血浆 1 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | TIMP2 |
| Protein aliases | CSC-21K, Tissue inhibitor of metalloproteinases 2, TIMP-2, Metalloproteinase inhibitor 2, testicular secretory protein Li 57 |
物种（Tested） | 人 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | CSC-21K, DDC8, TIMP2 |
| Gene ID | (Human) 7077 |
| Gene symbol | TIMP2 |
| UniProt ID | (Human) P16035 |
| The Human Tissue Inhibitor of MetalloProteinases 2 (TIMP-2) ELISA quantitates Hu TIMP-2 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TIMP-2.金属蛋白酶2（TIMP-2）（ELISA）可以定量人血清，血浆或细胞培养基中的人TIMP-2。 该检测试剂盒将专门识别天然和重组的人TIMP-2。Principle of the method 方法原理The Human TIMP-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人TIMP-2固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息TIMP-1 and TIMP-2 are inhibitory enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP-2 shows the highest binding affinity to both the latent (pro) and active forms of 72kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.TIMP-1和TIMP-2是基质金属蛋白酶家族的抑制酶，在维持结缔组织完整性方面非常重要。 TIMP-2对潜伏（原）和活性形式的72kDa IV型胶原酶（也称为MMP-2或明胶酶A）均显示出最高的结合亲和力。 它还对92kDa IV型胶原酶（也称为MMP-9或明胶酶B）的活性形式具有亲和力。 TIMP-2抑制肿瘤细胞和正常胎盘滋养层细胞的蛋白水解侵袭。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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