TNFRSF18-Cell lysates (GITR) Human ELISA Kit/人TNFRSF18-细胞裂解液（GITR）ELISA试剂盒
货号：EHTNFRSF18CL,EHTNFRSF18CLX5,EHTNFRSF18CLX10
规格：96 tests,5X96 tests,10X96 tests
价格：7238,29826,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 15 pg/mL |
| 检测范围 | 13.7-10,000 pg/mL |
样本类型/体积 | 细胞裂解液 20 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | GITR |
| Protein aliases | Activation-inducible TNFR family receptor, Glucocorticoid-induced TNFR-related protein, CD357, TNF receptor superfamily activation-inducible protein, Tumor necrosis factor receptor superfamily member 18, tumor necrosis factor receptor superfamily, member 18 |
物种（Tested） | 人 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | AITR, CD357, GITR, GITR-D, TNFRSF18, UNQ319/PRO364 |
| Gene ID | (Human) 8784 |
| Gene symbol | TNFRSF18 |
| UniProt ID | (Human) Q9Y5U5 |
| The Human TNFRSF18-Cell lysates (GITR) ELISA quantitates Hu GITR in human cell or tissue lysates. The assay will exclusively recognize both natural and recombinant Hu GITR.人TNFRSF18细胞裂解物（GITR）ELISA定量了人细胞或组织裂解物中的人GITR。 该检测试剂盒将专门识别天然和重组人GITR。Principle of the method 方法原理The Human GITR solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人GITR固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息CD357 (Glucocorticoid-induced tumor necrosis factor receptor family-related gene, GITR) is a member of the tumor necrosis factor receptor (TNFR) family that is expressed at low levels on unstimulated T cells, B cells, and macrophages. Upon activation, CD4+ and CD8+ T cells up-regulate CD357 expression, whereas immunoregulatory T cells constitutively express high levels of CD357.CD357（糖皮质激素诱导的肿瘤坏死因子受体家族相关基因，GITR）是肿瘤坏死因子受体（TNFR）家族的成员，在未刺激的T细胞，B细胞和巨噬细胞上低水平表达。 激活后，CD4 +和CD8 + T细胞上调CD357的表达，而免疫调节性T细胞组成性表达高水平的CD357。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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