p53 (Total) Human InstantOne ELISA Kit/人p53 (Total) InstantOne ELISA试剂盒
货号：85-86121-11
规格：96 tests
价格：6288
产品类型：InstantOne ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
样本类型/体积 | 细胞裂解液 20μL |
操作时间 | 1 hr 20 min |
测试时间 | 1 hr 10 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric microplate reader 酶标仪 |
规格 | 96 Tests |
组份 | - 捕获抗体 - 检测抗体 - InstantOne 检测板 - 细胞裂解液 - 增强剂溶液 - 洗涤液（10X） - 底物试剂（TMB） - 终止液 - 阳性对照细胞裂解物（冻干） - 密封条 |
储存温度 | 2-8℃ |
| Protein name | p53 |
物种（Tested） | 人 |
试剂盒类型 | InstantOne ELISA |
检测抗体偶联物 | Biotin |
标记（染料） | HRP |
| Gene ID | (Human) 7157 |
| Gene symbol | TP53 |
Gene aliases | BCC7, LFS1, P53, TRP53 |
UniProt ID | (Human) Q16848 |
| The InstantOne™ ELISA is specifically engineered for accurate measurement of total human p53 in cell lysates. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.InstantOne™ELISA是专门设计用于精确测量细胞裂解物中总人p53的。 InstantOne™ELISA试剂盒可在大约一小时内快速分析样品。 将传统的夹心ELISA中使用的所有试剂均以溶液形式添加到板上，然后进行洗涤步骤，并使用TMB比色底物进行检测。Target information 靶标信息The tumor suppressor protein, p53, is a sequence specific transcription factor that is activated by cellular stress. p53 mediates cell cycle arrest or apoptosis in response to DNA damage or starvation for pyrimidine nucleotides. p53 is up-regulated in response to stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization. p53 has been characterized to play a role in blocking the proliferative action of damaged cells and act as an anticancer agent. Phosphorylation of Ser392 in p53 has been shown to associate with the formation of human tumors. In addition, p53 has also been linked to the effects of aging and oxidative stress and an increase in p53 has been linked to deficits in LTP (Long Term Potentiation) in learning and memory. p53 is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families such as Li-Fraumeni syndrome.肿瘤抑制蛋白p53是一种序列特异性转录因子，可被细胞应激激活。 p53介导细胞周期停滞或凋亡，以响应嘧啶核苷酸的DNA损伤或饥饿。 p53响应应激信号而被上调，并被刺激激活特定基因的转录，从而导致p21waf1和其他参与G1或G2 / M阻滞的蛋白质的表达。 p53的结构包括N端反式激活结构域，中央DNA结合结构域，寡聚结构域和C端调节结构域。 p53上有多个磷酸化位点，其中Ser15处的磷酸化对于p53激活和稳定很重要。 p53的特征是在阻断受损细胞的增殖作用中起着作用，并起着抗癌剂的作用。已显示p53中Ser392的磷酸化与人类肿瘤的形成有关。此外，p53还与衰老和氧化应激的影响有关，p53的增加与学习和记忆中LTP（长期增强）的缺陷有关。在正常细胞中发现p53的水平非常低，但是，在多种转化的细胞系中，p53的表达量很高，据信有助于转化和恶性肿瘤。在许多不同的人类癌症中频繁出现的p53突变体无法结合共有DNA结合位点，并导致肿瘤抑制活性丧失。 TP53基因的改变不仅以人类恶性肿瘤的体细胞突变发生，而且以某些易发癌症的家族（例如李-弗劳梅尼综合症）的种系突变发生。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用，不用于诊断过程，未经明确授权不得转售。 |
| 数据： |
| p53 (Total) Human InstantOne™ ELISA KitRepresentative data for p53 (Total) Multispecies InstantOne™ ELISA. U2OS cells untreated (-) or treated with 10 ng/mL doxorubicin for 18 hours (+). Cells were analyzed by immunoblotting (left) and InstantOne™ ELISA (right). The intensity of the band at 53 kDa as detected by immunoblot correlates with InstantOne™ ELISA absorption values.p53（总）多物种InstantOne™ELISA的代表性数据。 未处理（-）或用10 ng / mL阿霉素处理18小时（+）的U2OS细胞。 通过免疫印迹（左）和InstantOne™ELISA（右）分析细胞。 通过免疫印迹检测到的53 kDa处的条带强度与InstantOne™ELISA吸收值相关。 |
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-20661.htm
QQ:1749072012