CREB (Phospho) [pS133] Multispecies InstantOne ELISA Kit/多物种CREB（磷酸）[pS133] InstantOne ELISA试剂盒
货号：85-86152-11
规格：96 tests
价格：0
产品类型：InstantOne ELISA
品牌：Thermo Fisher
物种：其它
宿主：其它
抗体亚型：其它
荧光染料：其它
样本类型/体积 | 细胞裂解液 20μL |
操作时间 | 1 hr 20 min |
测试时间 | 1 hr 10 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric microplate reader 酶标仪 |
规格 | 96 Tests |
组份 | - 捕获抗体 - 检测抗体 - InstantOne 检测板 - 细胞裂解液 - 增强剂溶液 - 洗涤液（10X） - 底物试剂（TMB） - 终止液 - 阳性对照细胞裂解物（冻干） - 密封条 |
储存温度 | 2-8℃ |
| Protein name | CREB |
物种（Tested） | 人 |
试剂盒类型 | InstantOne ELISA |
检测抗体偶联物 | Biotin |
标记（染料） | HRP |
| Gene ID | (Human) 1385 |
| Gene symbol | CREB1 |
Gene aliases | CREB, CREB-1 |
UniProt ID | (Human) P16220 |
| The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human, mouse, and hamster CREB (Ser133) in cell lysates. Detection of phosphorylated rat CREB (Ser133) is also expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.InstantOne™ELISA经过专门设计，可精确测量细胞裂解物中磷酸化的人，小鼠和仓鼠的CREB（Ser133）。 还期望检测到磷酸化的大鼠CREB（Ser133）。 InstantOne™ELISA试剂盒可在大约一小时内快速分析样品。 将传统的夹心ELISA中使用的所有试剂均以溶液形式添加到板上，然后进行洗涤步骤，并使用TMB比色底物进行检测。Target information 靶标信息CREB (Cyclic AMP response element binding protein) is a 43 kDa basic/leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation of CREB occurs via p44/42 MAP kinase and p90RSK, and via p38 MAP kinase and MSK1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133. CREB is involved in different cellular processes including the synchronization of circadian rhythmicity, differentiation of adipose cells, and learning and memory. In humans, the gene is located on the q arm of chromosome 2. Diseases associated with CREB dysfunction include Alzheimer’s Disease, histiocytoma and soft tissue melanoma.CREB（循环AMP反应元件结合蛋白）是一种43 kDa的碱性/亮氨酸拉链转录因子，与循环AMP反应元件（CRE）结合并响应各种细胞外信号（包括神经递质，激素，膜去极化和生长）激活转录和神经营养因子。 CREB的激活取决于丝氨酸133的磷酸化。CREB的磷酸化通过p44 / 42 MAP激酶和p90RSK以及通过p38 MAP激酶和MSK1发生。尽管CREB会结合DNA而不依赖于其磷酸化状态，但只有磷酸化形式才可以作为转录因子。 CREB结合蛋白（CBP）是一种与CREB直接相互作用的转录共激活因子，在丝氨酸133区域与CREB结合。CREB参与不同的细胞过程，包括昼夜节律的同步，脂肪细胞的分化以及学习和记忆。在人类中，该基因位于2号染色体的q臂上。与CREB功能障碍有关的疾病包括阿尔茨海默氏病，组织细胞瘤和软组织黑色素瘤。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用，不用于诊断过程，未经明确授权不得转售。 |
| 数据： |
| CREB (Phospho) [pS133] Multispecies InstantOne™ ELISA KitRepresentative data for CREB (Phospho) [pS133] Multispecies InstantOne™ ELISA. A431 cells were either treated with UCN-01 (1 µg/mL for 60 min) (-) or treated with EGF (2 µg/mL for 10 min) (+). Lysates were prepared as described in the InstantOne User Manual. Cell lysates were analyzed by both immunoblotting (left) and InstantOne™ ELISA (right) for the detection of phosphorylated CREB (Ser133). The intensity of the band at 37 kDa as detected by immunoblot correlates with InstantOne™ ELISA absorption values. CREB（磷酸）[pS133] Multispecies InstantOne™ELISA的代表性数据。 将A431细胞用UCN-01（1 µg / mL，持续60分钟）（-）或用EGF（2 µg / mL，持续10分钟）（+）处理。 如InstantOne用户手册中所述制备裂解液。 通过免疫印迹（左）和InstantOne™ELISA（右）对细胞裂解液进行分析，以检测磷酸化的CREB（Ser133）。 通过免疫印迹检测到的在37 kDa处的条带强度与InstantOne™ELISA吸收值相关。 |
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