GAPDH Human InstantOne ELISA Kit/人GAPDH InstantOne ELISA试剂盒
货号：85-86131-11
规格：96 tests
价格：0
产品类型：InstantOne ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
样本类型/体积 | 细胞裂解液 20μL |
操作时间 | 1 hr 20 min |
测试时间 | 1 hr 10 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric microplate reader 酶标仪 |
规格 | 96 Tests |
组份 | - 捕获抗体 - 检测抗体 - InstantOne 检测板 - 细胞裂解液 - 增强剂溶液 - 洗涤液（10X） - 底物试剂（TMB） - 终止液 - 阳性对照细胞裂解物（冻干） - 密封条 |
储存温度 | 2-8℃ |
| Protein name | GAPDH |
物种（Tested） | 人 |
试剂盒类型 | InstantOne ELISA |
检测抗体偶联物 | Biotin |
标记（染料） | HRP |
| Gene ID | (Human) 2597 |
| Gene symbol | GAPDH |
Gene aliases | G3PD, GAPD, HEL-S-162eP |
UniProt ID | (Human) P04406 |
| The InstantOne™ ELISA is specifically engineered for accurate measurement of human GAPDH in cell lysates. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.InstantOne™ELISA是专门设计用于精确测量细胞裂解液中人GAPDH的。 InstantOne™ELISA试剂盒可在大约一小时内快速分析样品。 将传统的夹心ELISA中使用的所有试剂均以溶液形式添加到板上，然后进行洗涤步骤，并使用TMB比色底物进行检测。Target information 靶标信息GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.GAPDH（甘油醛-3-磷酸脱氢酶）是一种通常被认为参与糖酵解的催化酶。 GAPDH以具有相同的37kDa亚基的四聚体形式存在，并在NADPH存在下催化将1,3-双磷酸甘油酸酯可逆还原为3-磷酸甘油醛。除了在糖酵解中起关键作用外，GAPDH还普遍表达并显示与其糖酵解功能无关的其他活性。据报道GAPDH参与DNA复制，DNA修复，核RNA输出，膜融合和微管捆绑的过程。研究提供了证据，证明GAPDH在凋亡中观察到的基因表达中起着至关重要的作用，并且是与年龄相关的神经退行性疾病的细胞表型的一部分。此外，GAPDH还参与了其他细胞过程，包括膜融合和癌症中的神经元凋亡。据报道，GAPDH与多种其他蛋白结合，包括淀粉样蛋白前体蛋白，导致某种形式的阿尔茨海默氏病（AD）的突变以及亨廷顿蛋白的多谷氨酰胺束，其蛋白产物异常形式是导致亨廷顿氏病的原因。还报道了GAPDH，肌动蛋白和微管蛋白之间的关联。由于GAPDH在大多数组织中均以高水平表达，因此在蛋白质印迹分析中可用作蛋白质负载控制。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用，不用于诊断过程，未经明确授权不得转售。 |
| 数据： |
| GAPDH Human InstantOne™ ELISA KitRepresentative data for GAPDH Multispecies InstantOne™ ELISA. HEK293 cells untreated (-) or treated with 2 µg/mL anisomycin for 30 min (+). Cells were analyzed by immunoblotting (left) and InstantOne™ ELISA (right). The intensity of the band at 37 kDa as detected by immunoblot correlates with InstantOne™ ELISA absorption values. GAPDH Multispecies InstantOne™ELISA的代表性数据。 未处理（-）或用2 µg / mL Anisomycin处理30分钟（+）的HEK293细胞。 通过免疫印迹（左）和InstantOne™ELISA（右）分析细胞。 通过免疫印迹检测到的在37 kDa处的条带强度与InstantOne™ELISA吸收值相关。 |
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