GSK3 beta (Total/Phospho) Multispecies InstantOne ELISA Kit/多物种GSK3 beta (Total/Phospho) InstantOne ELISA试剂盒
货号：85-86173-11
规格：96 tests
价格：0
产品类型：InstantOne ELISA
品牌：Thermo Fisher
物种：其它
宿主：其它
抗体亚型：其它
荧光染料：其它
样本类型/体积 | 细胞裂解液 20μL |
操作时间 | 1 hr 20 min |
测试时间 | 1 hr 10 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric microplate reader 酶标仪 |
规格 | 96 Tests |
组份 | - 捕获抗体 - 检测抗体 - InstantOne 检测板 - 细胞裂解液 - 增强剂溶液 - 洗涤液（10X） - 底物试剂（TMB） - 终止液 - 阳性对照细胞裂解物（冻干） - 密封条 |
储存温度 | 2-8℃ |
| Protein name | GSK3B |
物种（Tested） | 人，小鼠 |
试剂盒类型 | InstantOne ELISA |
检测抗体偶联物 | Biotin |
标记（染料） | HRP |
| Gene ID | (Mouse) 56637,(Human) 2932 |
| Gene symbol | Gsk3b, GSK3B |
Gene aliases | 7330414F15Rik, 8430431H08Rik, C86142, GSK-3, GSK-3beta, GSK3 |
UniProt ID | (Mouse) Q9WV60,(Human) P49841 |
| The InstantOne™ ELISA is specifically engineered for accurate measurement of total and phosphorylated human and mouse GSK3b (Ser9) in cell lysates. Detection of total and phosphorylated rat GSK3b (Ser9) is also expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.InstantOne™ELISA是专门设计用于精确测量细胞裂解物中总和磷酸化的人和小鼠GSK3b（Ser9）的方法。 还期望检测总的和磷酸化的大鼠GSK3b（Ser9）。 InstantOne™ELISA试剂盒可在大约一小时内快速分析样品。 将传统的夹心ELISA中使用的所有试剂均以溶液形式添加到板上，然后进行洗涤步骤，并使用TMB比色底物进行检测。Target information 靶标信息Glycogen synthase kinase-3 beta (GSK3 beta) belongs to the subfamily of glycogen synthase kinases, that play a central role in energy metabolism, neuronal cell development, and body pattern formation. GSK3 has alpha and beta isoforms (51 kDa and 47 kDa respectively), that are functionally dissimilar. GSK3 beta plays a role in insulin and Wnt signaling, and is inhibited following the extracellular signaling events.糖原合酶激酶3 beta（GSK3 beta）属于糖原合酶激酶的亚家族，在能量代谢，神经元细胞发育和身体形态形成中起着核心作用。 GSK3具有功能不同的α和β亚型（分别为51 kDa和47 kDa）。 GSK3 beta在胰岛素和Wnt信号传导中起作用，并在细胞外信号传导事件后被抑制。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization仅供研究使用，不用于诊断过程，未经明确授权不得转售。 |
| 数据： |
GSK3 beta (Total/Phospho) Multispecies InstantOne™ ELISA KitRepresentative data for GSK3 beta (Total/Phospho) Multispecies InstantOne™ ELISA. MCF7 cells were treated with either the PI3 Kinase inhibitor Wortmannin (10 µM) (-) or insulin (10 ng/mL for 10 min.) (+). Cell lysates were prepared as described in the InstantOne ELISA User Manual. Using the GSK3 beta (Total/Phospho) InstantOne ELISA, the cell lysates were analyzed for levels of Total GSK3 beta (left) and phospho-GSK3 beta (Ser9) (right).GSK3 beta（总/磷）多物种InstantOne™ELISA的代表性数据。 MCF7细胞用PI3激酶抑制剂Wortmannin（10 µM）（-）或胰岛素（10 ng / mL，持续10分钟）（+）处理。 如InstantOne ELISA用户手册中所述制备细胞裂解物。 使用GSK3 beta（Total / Phospho）InstantOne ELISA，分析了细胞裂解液的总GSK3 beta（左）和磷酸GSK3 beta（Ser9）（右）水平。 |
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-20687.htm
QQ:1749072012