p70 S6 Kinase (Phospho) [pT389] Multispecies InstantOne ELISA Kit/多物种p70 S6 Kinase (Phospho) [pT389] Multispecies InstantOne ELISA 试剂盒
货号：85-86052-11
规格：96 tests
价格：6634
产品类型：InstantOne ELISA
品牌：Thermo Fisher
物种：其它
宿主：其它
抗体亚型：其它
荧光染料：其它
样本类型/体积 | 细胞裂解液 20μL |
操作时间 | 1 hr 20 min |
测试时间 | 1 hr 10 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric microplate reader 酶标仪 |
规格 | 96 Tests |
组份 | - 捕获抗体 - 检测抗体 - InstantOne 检测板 - 细胞裂解液 - 增强剂溶液 - 洗涤液（10X） - 底物试剂（TMB - 终止液 - 阳性对照细胞裂解物（冻干） - 密封条 |
储存温度 | 2-8℃ |
| Protein name | p70 S6 Kinase |
物种（Tested） | 人，小鼠 |
试剂盒类型 | InstantOne ELISA |
检测抗体偶联物 | Biotin |
标记（染料） | HRP |
| Gene ID | (Mouse) 72508,(Human) 6198 |
| Gene symbol | Rps6kb1, RPS6KB1 |
Gene aliases | 2610318I15Rik, 4732464A07Rik, 70kDa, AA959758, AI256796, AI314060, S6K1, p70/85s6k, p70s6k, PS6K, S6K, S6K-beta-1, S6K1, STK14A, p70 S6KA, p70(S6K)-alpha, p70-S6K, p70-alpha |
UniProt ID | (Mouse) Q8BSK8,(Human) P23443 |
| The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human and mouse p70 S6K in cell lysates. Detection of rat p70 S6K is expected.The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.InstantOne™ELISA是专门设计用于准确测量细胞裂解物中磷酸化的人和小鼠p70 S6K的。 有望检测到大鼠p70S6K。InstantOne™ELISA试剂盒可在大约一小时内快速分析样品。 将传统的夹心ELISA中使用的所有试剂均以溶液形式添加到板上，然后进行洗涤步骤，并使用TMB比色底物进行检测。Target information 靶标信息The p70 S6 kinase (p70 Ribosomal Protein S6 Kinase, p70S6K) is a 70 kDa member of the ribosomal S6 kinase (RSK) family of serine/threonine kinases. p70 S6 kinase is predominantly localized in the cytoplasm, and is essential in growth factors regulated cell proliferation, pathways involving cell motility, such as metastases, the immune response, and tissue repair. p70 S6 kinase acts downstream of phosphoinositide (PI) 3-kinase, and its main physiological target is the S6 ribosomal protein, which is involved in upregulation of protein synthesis. The kinase activity of p70 S6 kinase leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding p70 S6 kinase and overexpression are seen in some breast cancer cell lines.p70 S6激酶（p70核糖体蛋白S6激酶，p70S6K）是丝氨酸/苏氨酸激酶的核糖体S6激酶（RSK）家族的70 kDa成员。 p70 S6激酶主要位于细胞质中，在调节细胞增殖的生长因子，涉及细胞运动的途径（如转移，免疫应答和组织修复）中至关重要。 p70 S6激酶在磷酸肌醇（PI）3-激酶的下游起作用，其主要生理靶点是S6核糖体蛋白，它参与蛋白合成的上调。 p70 S6激酶的激酶活性导致蛋白质合成和细胞增殖的增加。 在某些乳腺癌细胞系中发现了编码p70 S6激酶的DNA区域的扩增和过表达。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用，不用于诊断过程，未经明确授权不得转售。 |
| 数据： |
p70 S6 Kinase (Phospho) [pT389] Multispecies InstantOne™ ELISA KitRepresentative data for p70 S6 Kinase (Phospho) [pT389] Multispecies InstantOne™ ELISA. MCF7 cells were pre-treated with 10 µM wortmannin washed and unstimulated (-) or stimulated with 10 ng/mL insulin for 10 min (+). Cells were analyzed by immunoblotting (left) and InstantOne™ ELISA (right). The intensity of the band at 70 kDa as detected by immunoblot correlates with InstantOne™ ELISA absorption values.p70 S6激酶（磷酸）[pT389]多物种InstantOne™ELISA的代表性数据。 用10 µM渥曼青霉素预处理MCF7细胞，将其洗涤并未经刺激（-）或用10 ng / mL胰岛素刺激10分钟（+）。 通过免疫印迹（左）和InstantOne™ELISA（右）分析细胞。 通过免疫印迹检测到的在70 kDa处的条带强度与InstantOne™ELISA吸收值相关。 |
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