STAT5 alpha (Total/Phospho) [pY694/pY699] Human InstantOne ELISA Kit/人STAT5 alpha (Total/Phospho) [pY694/pY699] InstantOne ELISA试剂盒
货号：85-86113-11
规格：96 tests
价格：7593
产品类型：InstantOne ELISA
品牌：Thermo Fisher
物种：其它
宿主：其它
抗体亚型：其它
荧光染料：其它
样本类型/体积 | 细胞裂解液 20μL |
操作时间 | 1 hr 20 min |
测试时间 | 1 hr 10 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric microplate reader 酶标仪 |
规格 | 96 Tests |
组份 | - 捕获抗体 - 检测抗体 - InstantOne 检测板 - 细胞裂解液 - 增强剂溶液 - 洗涤液（10X） - 底物试剂（TMB - 终止液 - 阳性对照细胞裂解物（冻干） - 密封条 |
储存温度 | 2-8℃ |
| Protein name | STAT5 alpha |
物种（Tested） | 人 |
试剂盒类型 | InstantOne ELISA |
检测抗体偶联物 | Biotin |
标记（染料） | HRP |
| Gene ID | (Human) 6776 |
| Gene symbol | STAT5A |
Gene aliases | MGF, STAT5 |
UniProt ID | (Human) P42229 |
| The InstantOne™ ELISA is specifically engineered for accurate measurement of total and phosphorylated human STAT5A/B (Tyr694/Tyr699) in cell lysates. Detection of mouse and rat STAT5A/B is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.InstantOne™ELISA是专门设计用于精确测量细胞裂解液中总磷酸化人STAT5A / B（Tyr694 / Tyr699）的试剂盒。 预计会检测到小鼠和大鼠的STAT5A / B。 InstantOne™ELISA试剂盒可在大约一小时内快速分析样品。 将传统的夹心ELISA中使用的所有试剂均以溶液形式添加到板上，然后进行洗涤步骤，并使用TMB比色底物进行检测。Target information 靶标信息STAT5 alpha is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5 alpha is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of STAT5 alpha in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of STAT5 alpha is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this protein in cells. STAT5 alpha, along with STAT5 beta, were identified in mouse. The amino acid sequences of STAT5 alpha and STAT5 beta show 96% sequence similarity, and both proteins are co expressed in most tissues in virgin and lactating mice. However, differential accumulation of STAT5 alpha and STAT5 beta mRNA has been reported for both muscle and mammary tissue. STAT5 alpha is critically involved in a variety of physiological functions, including reproduction, lactation, immune function and somatic growth.STAT5 alpha是STAT转录因子家族的成员。响应细胞因子和生长因子，STAT家族成员被受体相关的激酶磷酸化，然后形成同二聚体或异二聚体，这些同二聚体或异二聚体易位至细胞核，并在其中充当转录激活因子。 STAT5α被许多细胞配体（例如IL2，IL3，IL7 GM-CSF，促红细胞生成素，血小板生成素和不同的生长激素）激活并介导其响应。与TEL / JAK2基因融合相关的骨髓瘤和淋巴瘤中STAT5α的激活与细胞刺激无关，并且已被证明对肿瘤发生至关重要。发现STAT5 alpha的小鼠对应物诱导BCL2L1 / BCL-X（L）的表达，这提示该蛋白在细胞中具有抗凋亡功能。在小鼠中鉴定出STAT5α以及STAT5β。 STAT5 alpha和STAT5 beta的氨基酸序列显示96％的序列相似性，并且这两种蛋白质在处女和哺乳期小鼠的大多数组织中共表达。然而，据报道，肌肉和乳腺组织中STAT5α和STAT5βmRNA的差异积累。 STAT5α关键参与多种生理功能，包括生殖，泌乳，免疫功能和体细胞生长。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用，不用于诊断过程，未经明确授权不得转售。 |
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