IL-5 Mouse Uncoated ELISA Kit with Plates/小鼠IL-5 ELISA试剂盒含未包被板
货号：88-7054-22,88-7054-86,88-7054-76
规格：2X96 Tests,10X96 Tests,20X96 Tests
价格：3125,6537,9393
产品类型：自定义ELISA
品牌：Thermo Fisher
物种：小鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 8 pg/mL |
| 检测范围 | 8-1,000 pg/mL |
样本类型/体积 | 血浆 20 μL 血清 20 μL 上清液 100 μL |
操作时间 | 1 hr 45 min |
测试时间 | 24 hr 30 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 2 X 96 Tests |
组份 | - 捕获抗体：Pre-titrated, 纯化抗体 - 检测抗体：Pre-titrated, Biotin偶联抗体 - 标准品：重组细胞因子，用于生成标准曲线和校准样品 - 包被缓冲液：10X PBS ELISA包被缓冲液 - 检测稀释液：5X浓缩液 - 检测酶：Pre-titrated Avidin-HRP - 底物溶液：Tetramethylbenzidine (TMB)底物溶液 - 分析证书：特定批次说明书用于抗体和标准品稀释 - 96孔板：Corning Costar 9018（含产品目录号，后缀 -22，-76，-86） |
储存温度 | 2-8℃ |
| Protein name | TGF beta-1 |
| Protein aliases | LAP, Latency-associated peptide, prepro-transforming growth factor beta-1, regulatory protein, TGF-beta 1, TGF-beta-1, Transforming growth factor beta-1, Transforming growth factor beta-1 proprotein, transforming growth factor-beta 1 |
物种（Tested） | 小鼠，人 |
试剂盒类型 | Uncoated ELISA Kit with Plates |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene ID | (Human) 7040,(Mouse) 21803 |
| Gene aliases | CED, DPD1, LAP, TGF-beta1, TGFB, Tgfb-1, TGFB1, TGFbeta, TGFbeta1 |
| Gene symbol | TGFB1, Tgfb1 |
UniProt ID | (Human) P01137,(Mouse) P04202 |
| The Mouse Interleukin-5 (IL-5) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IL-5. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.小鼠白介素5（IL-5）未包被的ELISA试剂盒包含预先匹配的抗体对，板和试剂，用于进行定量酶联免疫吸附测定（ELISA），以检测和定量小鼠IL-5的蛋白质水平。 需要洗涤缓冲液和终止液才能完成ELISA反应，需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA被设计为测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被到微孔板孔的底部，这是一整夜的过程。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. IL-5 cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of IL-5 cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). IL-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. IL-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.IL-5是一种细胞因子，可同时充当B细胞和嗜酸性粒细胞的生长和分化因子。 IL-5细胞因子是嗜曙红细胞生成，嗜酸性粒细胞成熟和激活的主要调节因子。 据报道，这种细胞因子的产生增加与哮喘或嗜酸性粒细胞增多综合征有关。 IL-5细胞因子的受体是异二聚体，其β亚基与白细胞介素3（IL3）和集落刺激因子2（CSF2 / GM-CSF）的受体共有。 IL-5与白介素4（IL4），白介素13（IL13）和CSF2一起，在5号染色体上形成细胞因子基因簇。发现IL-5，IL4和IL13受长距离协同调节。 调控元件在5q31号染色体上分布了120千个碱基。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-20708.htm
QQ:1749072012