IL-1 alpha Mouse Uncoated ELISA Kit/小鼠IL-1 alpha未包被ELISA试剂盒
货号：88-5019-88,88-5019-77
规格：10X96 Tests,20X96 Tests
价格：6261,8846
产品类型：自定义ELISA
品牌：Thermo Fisher
物种：小鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 4 pg/mL |
| 检测范围 | 4-500 pg/mL |
样本类型/体积 | 血浆 100 μL 血清 100 μL 上清液 100 μL |
操作时间 | 1 hr 45 min |
测试时间 | 24 hr 30 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 10 X 96 Tests |
组份 | - 捕获抗体：Pre-titrated, 纯化抗体 - 检测抗体：Pre-titrated, Biotin偶联抗体 - 标准品：重组细胞因子，用于生成标准曲线和校准样品 - 包被缓冲液：10XPBS ELISA包被缓冲液 - 检测稀释液：5X浓缩液 - 检测酶：Pre-titrated Avidin-HRP - 底物溶液：TMB溶液 - 分析证书：特定批次说明书用于抗体和标准品稀释 - 96孔板：Corning Costar flat-bottom plates（含产品目录号，后缀 -22，-76，-86） |
储存温度 | 2-8℃ |
| Protein name | IL-1 alpha |
| Protein aliases | IL-1 alpha, Interleukin-1 alpha |
物种（Tested） | 小鼠 |
试剂盒类型 | Uncoated ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | Il-1a, Il1a |
| Gene ID | (Mouse) 16175 |
| Gene symbol | Il1a |
| UniProt ID | (Mouse) P01582 |
| The Mouse Interleukin-1alpha (IL-1 α) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IL-1 α. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.小鼠白介素-1α（IL-1α）未包被的ELISA试剂盒包含预先匹配的抗体对，以及用于执行定量酶联免疫吸附测定（ELISA）的试剂，以检测和定量小鼠IL-1α的蛋白质水平。 需要洗涤缓冲液和终止液才能完成ELISA反应，需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA被设计为测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被到微孔板孔的底部，这是一整夜的过程。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息IL-1 alpha (Interleukin-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 alpha is coded by the IL1A gene and signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 beta. IL1A is located on the q arm on chromosome 2 at position 13. IL-1 alpha plays an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. IL-1 alpha activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. Further, IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury and induces apoptosis. The IL1A gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. IL1 alpha regulates the activities of NF-kappaB and mitogen-activated protein kinases. IL-1 alpha can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-1 alpha are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.IL-1 alpha（白介素-1 alpha）是一种由单核细胞，巨噬细胞和树突状细胞表达的促炎细胞因子。 IL-1 alpha由IL1A基因编码，并通过两个受体IL-1RI和IL-1RII发出信号，这两个受体均与IL-1 beta共享。 IL1A位于13号染色体2的q臂上。IL-1alpha通过触发靶细胞中其他促炎细胞因子的产生并启动急性期反应，在先天宿主防御中发挥重要作用。 IL-1受体拮抗剂（IL-1RA）可以调节IL-1α的活性，IL-1RA是许多细胞类型产生的一种蛋白质，通过竞争性抑制作用阻断受体结合。 IL-1α是参与多种免疫反应，炎症过程和造血作用的多效细胞因子。此外，IL-1α由单核细胞和巨噬细胞产生为前蛋白，其被蛋白水解加工并响应细胞损伤而释放并诱导凋亡。 IL1A基因和其他8个白介素1家族基因在2号染色体上形成一个细胞因子基因簇。IL1α调节NF-κB和丝裂原激活的蛋白激酶的活性。 IL-1α可以刺激IL6和环氧合酶2（PTGS2 / COX-2）的表达，并增强一氧化氮（NO）的产生。高水平的IL-1α与几种慢性炎症疾病有关，包括类风湿关节炎，牛皮癣和多发性硬化症。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。 |
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-20717.htm
QQ:1749072012