TGFb1/LAP Human Uncoated ELISA Kit with Plates/人TGFb1/LAP ELISA试剂盒含未包被板
货号：88-50390-76,88-50390-86,88-50390-22
规格：20X96 Tests,10X96 Tests,2X96 Tests
价格：9393,6537,3125
产品类型：自定义ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 156.3 pg/mL |
| 检测范围 | 156.3-10,000 pg/mL |
样本类型/体积 | 血浆 10 μL 血清 10 μL 上清液 10 μL |
操作时间 | 1 hr 45 min |
测试时间 | 24 hr 30 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 20 X 96 Tests |
组份 | - 捕获抗体：Pre-titrated, 纯化抗体 - 检测抗体：Pre-titrated, Biotin偶联抗体 - 标准品：重组细胞因子，用于生成标准曲线和校准样品 - 10X包被缓冲液：用于包被捕获抗体的缓冲液 - 5XELISA/ELISPOT稀释液：封闭和稀释检测抗体和酶的缓冲液 - 检测酶：Pre-titrated Avidin-HRP - 底物：1X TMB溶液 - 分析证书：特定批次说明书用于抗体和标准品稀释 - 96孔板：Corning Costar 9018（含产品目录号，后缀 -22，-76，-86） |
储存温度 | 2-8℃ |
| Protein name | TGF beta-1 |
| Protein aliases | LAP, Latency-associated peptide, prepro-transforming growth factor beta-1, TGF-beta-1, Transforming growth factor beta-1, Transforming growth factor beta-1 proprotein |
物种（Tested） | 人 |
试剂盒类型 | Uncoated ELISA Kit with Plates |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene ID | (Human) 7040 |
| Gene aliases | CED, DPD1, LAP, TGFB, TGFB1, TGFbeta |
| Gene symbol | TGFB1 |
UniProt ID | (Human) P01137 |
| The Human Transforming Growth Factor beta 1/LAP/CD276 Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human TGFβ1. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.人转化生长因子beta 1 / LAP / CD276未包被ELISA试剂盒包含预先匹配的抗体对，板和试剂，用于进行定量酶联免疫吸附测定（ELISA），以检测和定量人类TGFβ1的蛋白质水平。 需要洗涤缓冲液和终止液才能完成ELISA反应，需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA被设计为测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被到微孔板孔的底部，这是一整夜的过程。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息TGF beta-1 (TGFB1, Transforming Growth Factor Beta 1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines, found almost ubiquitously in tissues. Transforming growth factor (TGF)-b is stored in the extracellular matrix as a latent complex with its pro-domain. Activation of TGF beta-1 requires the binding of aV integrin to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-b binding proteins. Latent forms are complexes of TGF beta-1, an amino-terminal portion of the TGF-beta precursor, designated TGF-LAP (TGF-latency associated peptide), and a specific binding protein, known as LTBP. TGF beta-1 helps regulates proliferation, differentiation, adhesion, migration in many cell types. Many cells have TGF beta receptors, and the protein positively and negatively regulates many other growth factors. TGF beta-1 is cleaved into a latency-associated peptide and a mature TGF beta-1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGF beta-1 homodimer. The mature peptide may also form heterodimers with other TGF beta family members. The gene for TGF beta-1 is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease and cystic fibrosis.TGF beta-1（TGFB1，转化生长因子Beta 1）是细胞因子转化生长因子β超家族的多肽成员，几乎在组织中普遍存在。转化生长因子（TGF）-b作为潜在复合物以其前结构域形式存储在细胞外基质中。 TGFβ-1的激活需要aV整联蛋白与前结构域中的RGD序列结合，并在该结构域上施加作用力，该作用域由潜在的TGF-b结合蛋白保持在细胞外基质中。潜在形式是TGFβ-1，TGF-β前体的氨基末端部分，称为TGF-LAP（TGF-潜伏期相关肽）和特定结合蛋白（称为LTBP）的复合物。 TGF beta-1有助于调节许多细胞类型的增殖，分化，粘附，迁移。许多细胞具有TGFβ受体，并且该蛋白可以正负调节许多其他生长因子。 TGFβ-1裂解为潜伏期相关肽和成熟的TGFβ-1肽，并以由TGFB1同型二聚体，LAP同型二聚体和潜在TGFB1结合蛋白组成的潜伏形式存在，或在活性形式由TGFβ-1同型二聚体组成。成熟的肽还可以与其他TGFβ家族成员形成异二聚体。 TGFβ-1的基因在肿瘤细胞中经常被上调，该基因的突变导致Camurati-Engelmann病和囊性纤维化。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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