IgM Rat Uncoated ELISA Kit/大鼠IgM未包被ELISA试剂盒
货号：88-50540-88,88-50540-77
规格：10X96 Tests,20X96 Tests
价格：6261,8846
产品类型：自定义ELISA
品牌：Thermo Fisher
物种：大鼠
宿主：其它
抗体亚型：IgM
荧光染料：其它
灵敏度 | 0.3125 ng/mL |
| 检测范围 | 0.3125-20 ng/mL |
样本类型/体积 | 血浆 10 μL 血清 10 μL 上清液 10 μL |
操作时间 | 1 hr 45 min |
测试时间 | 24 hr 30 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 10 X 96 Tests |
组份 | - 捕获抗体：Pre-titrated, 纯化抗体 - 检测抗体：Pre-titrated, HRP偶联抗体 - 标准品：重组细胞因子，用于生成标准曲线和校准样品 - 10X包被缓冲液：用于包被捕获抗体的缓冲液 - 5XELISA/ELISPOT稀释液：封闭和稀释检测抗体和酶的缓冲液 - 底物：1X TMB溶液 - 分析证书：特定批次说明书用于抗体和标准品稀释 - 96孔板：Corning Costar 9018（含产品目录号，后缀 -22，-76，-86） |
储存温度 | 2-8℃ |
| Protein name | Rat IgM |
物种（Tested） | 大鼠 |
试剂盒类型 | Uncoated ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | HRP |
| The Rat IgM Uncoated ELISA Kit contains pre-matched antibody pairs and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of rat IgM. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.大鼠IgM未包被ELISA试剂盒包含预先匹配的抗体对和试剂，用于执行定量酶联免疫吸附测定（ELISA），以检测和定量大鼠IgM的蛋白质水平。 需要洗涤缓冲液和终止液才能完成ELISA反应，需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA被设计为测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被到微孔板孔的底部，这是一整夜的过程。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息Immunoglobulin M is the third most common serum Ig and can exist as pentamer where all heavy chains are identical and all light chains are identical or as a monomer (e.g. found on B lymphocytes as B - cell receptors). The large pentameric structure allows for building of bridges between encountered epitopes on molecules that are too distant as to be connected by smaller IgG antibodies. IgM is built as the first antibody during an immune response and is responsible for agglutination and cytolytic reactions since its pentameric structure gives it 10 free antigen binding sites in theory and it posseses a high avidity. Due to conformational constraints among the 10 Fab portions, IgM only has a valence of 5. Additionally, IgM is not as versatile as IgG, but it is of vital importance in complement activation and agglutination. IgM is predominantly found in the lymph fluid and the blood and is a very effective neutralizing agent in the early stages of disease. Elevated levels can be a sign of recent infection or exposure to antigen.免疫球蛋白M是第三大最常见的血清Ig，可以以五聚体形式存在，其中所有重链均相同，所有轻链均相同或以单体形式存在（例如在B淋巴细胞上作为B细胞受体存在）。大的五聚体结构允许在分子上遇到的表位之间建立桥梁，这些表位距离太远，无法通过较小的IgG抗体连接。 IgM被构建为免疫应答期间的第一抗体，并负责凝集和细胞溶解反应，因为其五聚体结构在理论上为它提供了10个自由抗原结合位点，并且具有很高的亲和力。由于10个Fab部分之间的构象限制，IgM的化合价仅为5。此外，IgM的功能不如IgG，但对补体激活和凝集至关重要。 IgM主要存在于淋巴液和血液中，是疾病早期的一种非常有效的中和剂。水平升高可能是近期感染或暴露于抗原的迹象。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。 |
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