MCP-1/CCL2 Mouse Uncoated ELISA Kit/小鼠MCP-1/CCL2 未包被ELISA试剂盒
货号：88-7391-88,88-7391-77
规格：10X96 Tests,20X96 Tests
价格：6261,8846
产品类型：自定义ELISA
品牌：Thermo Fisher
物种：小鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 15 pg/mL |
| 检测范围 | 15-2,000 pg/mL |
样本类型/体积 | 血浆 100 μL 血清 100 μL 上清液 100 μL |
操作时间 | 1 hr 45 min |
测试时间 | 24 hr 30 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 10 X 96 Tests |
组份 | - 捕获抗体：Pre-titrated, 纯化抗体 - 检测抗体：Pre-titrated, Biotin偶联抗体 - 标准品：重组细胞因子，用于生成标准曲线和校准样品 -10X包被缓冲液：用于包被捕获抗体 - 5X ELISA/ELISPOT 稀释液：用于稀释检测抗体和酶得缓冲液 - 检测酶：Pre-titrated Avidin-HRP - 底物溶液：1X TMB溶液 - 分析证书：特定批次说明书用于抗体和标准品稀释 - 96孔板：Corning Costar 9018（含产品目录号，后缀 -22，-76，-86） |
储存温度 | 2-8℃ |
| Protein name | CCL2 (MCP-1) |
| Protein aliases | C-C motif chemokine 2, MCP-1, Monocyte chemoattractant protein 1, monocyte chemoattractant protein-1, Monocyte chemotactic protein 1, Platelet-derived growth factor-inducible protein JE, small inducible cytokine A2, Small-inducible cytokine A2 |
物种（Tested） | 小鼠 |
试剂盒类型 | Uncoated ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene ID | (Mouse) 20296 |
| Gene aliases | AI323594, Ccl2, HC11, JE, MCAF, MCP-1, MCP1, Scya2, Sigje, SMC-CF |
| Gene symbol | Ccl2 |
UniProt ID | (Mouse) P10148 |
| The Mouse Monocyte Chemoattractant Protein-1/CCL2 (MCP-1) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse MCP-1. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.小鼠单核细胞趋化蛋白-1 / CCL2（MCP-1）未包被的ELISA试剂盒包含预匹配的抗体对，以及用于进行定量酶联免疫吸附测定（ELISA）的试剂，以检测和定量小鼠MCP-1的蛋白水平。 需要洗涤缓冲液和终止液才能完成ELISA反应，需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA被设计为测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被到微孔板孔的底部，这是一整夜的过程。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息MCP-1 (Monocyte chemotactic protein-1, CCL2) has a predicted molecular weight of approximately 11kDa, however, it may run at a higher molecular weight due to glycosylation. MCP-1 is a member of the cytokine family and plays an important role in immunoregulation and inflammatory processes. MCP-1 signals through G protein-coupled receptors, CCR2 and CCR4, to recruit memory T cells, monocytes and dendritic cells. MCP-1 is linked with tumor progression, angiogenesis, and displays selective chemotactic activity towards monocytes and basophils but not neutrophils or eosinophils. MCP-1 is produced by a variety of cell types and is a potent chemoattractant for monocytes, memory T lymphocytes, and natural killer (NK) cells. Further, MCP-1 is indicated in the etiology of several immune disorders, and is upregulated during infection and inflammation. While MCP-1 is a potent basophil activator, the related protein MCP-2 stimulates both eosinophils and basophils. MCP-3 has been shown to have the broadest range of influence. MCP-1 has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis.MCP-1（单核细胞趋化蛋白-1，CCL2）的预测分子量约为11kDa，但是由于糖基化，其分子量可能更高。 MCP-1是细胞因子家族的成员，在免疫调节和炎症过程中起重要作用。 MCP-1通过G蛋白偶联受体CCR2和CCR4发出信号，以募集记忆T细胞，单核细胞和树突状细胞。 MCP-1与肿瘤进展，血管生成有关，并显示出对单核细胞和嗜碱性粒细胞的选择性趋化活性，但对嗜中性粒细胞或嗜酸性粒细胞没有选择性。 MCP-1由多种细胞类型产生，是单核细胞，记忆T淋巴细胞和自然杀伤（NK）细胞的有效化学引诱剂。此外，MCP-1在几种免疫疾病的病因学中有指示，并且在感染和炎症过程中被上调。虽然MCP-1是有效的嗜碱性粒细胞活化剂，但相关蛋白MCP-2刺激嗜酸性粒细胞和嗜碱性粒细胞。已经证明MCP-3具有最广泛的影响力。 MCP-1已经牵涉到以单核细胞浸润为特征的疾病的发病机理，例如牛皮癣，类风湿性关节炎或动脉粥样硬化。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。 |
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