IL-15/IL-15R Complex Mouse Uncoated ELISA Kit/小鼠IL-15/IL-15R未包被ELISA试剂盒
货号：88-7215-88
规格：10 x 96 tests
价格：6261
产品类型：自定义ELISA
品牌：Thermo Fisher
物种：小鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 4 pg/mL |
| 检测范围 | 4-500 pg/mL |
样本类型/体积 | 血清 100 μL 上清液 100 μL |
操作时间 | 1 hr 45 min |
测试时间 | 24 hr 30 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 10X 96 Tests |
组份 | - 捕获抗体：Pre-titrated, 纯化抗体 - 检测抗体：Pre-titrated, Biotin偶联抗体 - 标准品：重组细胞因子，用于生成标准曲线和校准样品 -ELISA/ELISPOT包被缓冲液：10X PBS ELISA包被缓冲液 - 检测稀释液：5X浓缩液 - 检测酶：Pre-titrated Avidin-HRP - 底物溶液：Tetramethylbenzidine (TMB) 溶液 - 分析证书：特定批次说明书用于抗体和标准品稀释 - 96孔板：Corning Costar Flat-bottom（含产品目录号，后缀 -22，-86） |
储存温度 | 2-8℃ |
| Protein name | IL-15/IL-15R |
| Protein aliases | CD215, IL-15, IL-15 receptor subunit alpha, IL-15R-alpha, Interleukin-15, Interleukin-15 receptor subunit alpha, sIL-15 receptor subunit alpha, Soluble interleukin-15 receptor subunit alpha |
物种（Tested） | 小鼠 |
试剂盒类型 | Uncoated ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | AA690181, AI503618, IL-15, IL-15RA, Il15, Il15ra |
| Gene ID | (Mouse) 16168,(Mouse) 16169 |
| Gene symbol | Il15, Il15ra |
| UniProt ID | (Mouse) P48346,(Mouse) Q60819 |
| The Mouse Interleukin 15/IL-15 Receptor Complex (IL-15/IL-15R) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IL-15/IL-15R. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.小鼠白介素15 / IL-15受体复合物（IL-15 / IL-15R）未包被的ELISA试剂盒包含预匹配的抗体对以及用于进行定量酶联免疫吸附测定（ELISA）的试剂，以检测和定量小鼠IL的蛋白质水平 -15 / IL-15R。 需要洗涤液和终止液才能完成ELISA反应，需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA是测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被到微孔板孔的底部，这是一整夜的过程。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息Interleukin-15 (IL-15) is a 14 kDa proinflammatory protein that has been shown to play a role in the activation of neutrophils, dendritic cells, and macrophages, and is essential to the development and survival of NK cells and CD8 T-cells. IL-15 activates Jak/Stat pathways by signaling through a heterotrimeric receptor. Two of the subunits of this receptor, beta and gamma, are shared with the IL-2 receptor, while the alpha subunit is unique to IL-15.Despite the expression of IL-15 mRNA in many cell types, the secreted protein is rarely detectable in biological samples. Recent research suggests that IL-15 is retained inside the cell and is only secreted in complex with its unique receptor, IL-15R alpha. This chaperoning is required from the ER to Golgi through to secretion. The cytokine/receptor complex may stay bound to the cell surface, where it can be trans-presented to cells expressing the beta/gamma subunits of the receptor, or may be secreted in a soluble form that lacks the transmembrane domain of the receptor. The soluble IL-15/IL-15R complex is produced by mouse dendritic cells, fibroblasts, and macrophages, and demonstrates a 10-100-fold increase in agonistic activity over IL-15 alone.白介素15（IL-15）是一种14 kDa的促炎蛋白，已显示在嗜中性粒细胞，树突状细胞和巨噬细胞的活化中起作用，并且对NK细胞和CD8 T细胞的发育和存活至关重要。 IL-15通过异源三聚体受体发出信号来激活Jak / Stat途径。该受体的两个亚基β和γ与IL-2受体共有，而α亚基是IL-15所特有的。尽管IL-15 mRNA在许多细胞类型中都有表达，但分泌的蛋白很少在生物样品中可检测到。最近的研究表明，IL-15保留在细胞内部，仅以其独特的受体IL-15Rα的复合体形式分泌。从ER到高尔基体直至分泌都需要这种陪伴。细胞因子/受体复合物可以保持与细胞表面的结合，在这里它可以被转递到表达受体β/γ亚基的细胞上，或者可以以缺乏受体跨膜结构域的可溶形式被分泌。可溶性IL-15 / IL-15R复合物是由小鼠树突状细胞，成纤维细胞和巨噬细胞产生的，与单独的IL-15相比，其激动活性提高了10-100倍。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。 |
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