TNF alpha Mouse Instant ELISA Kit/小鼠TNF α一步法ELISA试剂盒
货号：BMS607-2INST
规格：128 tests
价格：6668
产品类型：Instant ELISA
品牌：Thermo Fisher
物种：小鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度： | 4.0 pg/mL | 产品规格： | 128 tests |
检测范围： | 31.3-2,000 pg/mL | 标记染料： | HRP |
仪器： | 比色酶标仪 | 储存： | -20°C |
操作时间： | 20 min | 蛋白名称： | TNF alpha |
测试时间： | 3 hr 10 min | 特异性靶标位点： | |
一致性（未洗涤）： | No | 物种（经过测试）： | Mouse |
批间变异系数： | 9.8% | 检测试剂盒类型： | 即用型ELISA 试剂盒 |
批内变异系数： | 7.2% | 检测抗体偶联物： | Biotin |
| 同名基因： | DIF, TNF-a, TNF-alpha, TNFSF2, TNFalpha, Tnfa, Tnfsf1a | 基因名称： | Tnf |
样品类型/体积： | 血清 50 µL 血浆 50µL 上清液 50µL | 组分： | - 预包被96孔板 - 生物素化检测抗体 - 链霉亲和素-辣根过氧化物酶 - 样品稀释液，冻干 - 标准曲线条 - 样品稀释液 - 洗涤浓缩液 - 底物溶液 - 终止液 - 裂解液10X - 胶板盖 |
| The Mouse Tumor Necrosis Factor alpha (Ms TNFα) ELISA quantitates Ms TNFα in mouse serum, plasma, cell culture supernatants or other body fluids. The assay will exclusively recognize both natural and recombinant Ms TNFα.小鼠肿瘤坏死因子α（MsTNFα）ELISA定量检测小鼠血清，血浆，细胞培养上清液或其他体液中的MsTNFα。 该检测试剂盒将专门识别天然和重组MsTNFα。Principle of the method 方法原理The Mouse TNFα solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.小鼠TNFα夹心ELISA（酶联免疫吸附测定）是测量匹配的抗体对之间结合的靶标量。 已在孔中预包被了靶标特异性抗体。 将样品或对照添加到这些孔中，并与固定的（捕获）抗体结合。 通过将第二（检测）抗体与捕获抗体不同表位上的靶标结合形成三明治。 与酶结合的抗体结合形成的三明治。 经过孵育和洗涤步骤以去除微孔板中未结合的物质后，添加底物溶液，该溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Tumor Necrosis Factor is a polypeptide cytokine produced by monocytes and macrophages. It functions as a multipotent modulator of immune response and further acts as a potent pyrogen. TNF-alpha circulates throughout the body responding to stimuli (infectious agents or tissue injury), activating neutrophils, altering the properties of vascular endothelial cells, regulating metabolic activities of other tissues, as well as exhibiting tumoricidal activity by inducing localized blood clotting. TNF-alpha also inhibits lipoprotein lipase activity resulting in cachexia, a physical wasting condition. Activation of B-cells by the Epstein Barr virus can be inhibited by TNF-alpha. Due to its varied actions throughout the immune system, TNF-alpha may play a role in the pathogenesis of many disease states.肿瘤坏死因子是单核细胞和巨噬细胞产生的多肽细胞因子。 它起着免疫反应多能调节剂的作用，并进一步起着有效的热原作用。 TNF-α在全身循环，对刺激（感染因子或组织损伤）作出反应，激活中性粒细胞，改变血管内皮细胞的特性，调节其他组织的代谢活性，并通过诱导局部凝血而表现出杀肿瘤活性。 TNF-α还抑制脂蛋白脂肪酶的活性，导致恶病质，一种物理上的浪费状态。 爱泼斯坦巴尔病毒对B细胞的激活可以被TNF-α抑制。 由于其在整个免疫系统中的不同作用，TNF-α可能在许多疾病状态的发病机理中起作用。TNF-alpha production is mediated by the action of lymphokines and endotoxins on the macrophage. Purified monocytes produce TNF-alpha within four hours of stimulation by recombinant IL-2 and there is some in vitro evidence to suggest that TNF-alpha is expressed at high levels and with prolonged kinetics in T cells stimulated by both CD2 and CD28. Secretion of TNF-alpha is enhanced by gamma interferon. TNF then induces or enhances the specific production of Class I MHC antigen, GM-CSF, and IL-1.TNF-α的产生是通过巨噬细胞上淋巴因子和内毒素的作用介导的。 纯化的单核细胞在重组IL-2刺激的四个小时内产生TNF-α，并且有一些体外证据表明TNF-α在CD2和CD28刺激的T细胞中高水平表达，并且动力学延长。 γ-干扰素可增强TNF-α的分泌。 然后，TNF诱导或增强I类MHC抗原，GM-CSF和IL-1的特异性产生。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
| 特点： |
| -极其简便快捷：操作时间仅15 min- 使用率更高：单独的标品板条，可检测96个样品- 适用客户：高度追求实验效率的客户 |
| 数据： |
TNF alpha Mouse Instant ELISA™ KitRepresentative standard curve for Mouse TNF alpha Instant ELISA |
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