TAMRA Synthesis Column (5'-DMT-mdC(TEG-TAMRA)-Phos-CPG); 1000 Å
货号：CG1-5008-1,CG1-5008-2,CG1-5008-5
规格：1 µmol,200 nmol,50 nmol
价格：1266.3,351.75,211.05
产品类型：核酸合成
品牌：LGC Biosearch Technologies
| Biosearch Technologies' 5'-DMT-mdC(TEG-TAMRA)-Phosphate CPG is based on a modified cytosine residue linked to the TAMRA moiety via a triethyleneglycol spacer. The 3' end is conjugated to the CPG support via a phosphate linkage. Our TAMRA CPG supports allow for the introduction of a reporter or quencher molecule onto the 3'-terminus end of the oligonucleotide. |
| 特性： |
| ▪ Formula:C77H91N8O21PS▪ Molecular Weight:1527.63▪ Appearance:pink powder▪ Absorption Maximum (Lambda Max):555▪ Extinction Coefficient at Lambda max:90000▪ Extinction Coefficient at 260 nm:31980▪ Fluorescence Maximum:576 |
| 产品用途： |
| Synthesis conditions:Synthesis can be performed on these supports according to standard procedures with deprotection and cleavage modifications noted.Cleavage conditions:Use t-butylamine/water (1:3) for 45 minutes at 25 °C. The use of ammonia or methylamine must be avoided, as it will cause degradation of the TAMRA moiety.Deprotection conditions:When using fast deprotecting amidites (eg. C-Ac, G-DMF, G-PAC) use t-butylamine/Water (1:3) for 6 hours at 60° C. When using standard amidites (eg. C-Bz, G-iBu) use t-butylamine/Water (1:3) overnight (12-15 hours) at 60°C. Image of cleaved and deprotected structure:The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is: 998.91 |
| 数据： |
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