IL-4 Human Instant ELISA Kit/人IL-4一步法ELISA试剂盒
货号：BMS225INST
规格：128 tests
价格：6668
产品类型：Instant ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度： | 0.66 pg/mL | 产品规格： | 128 tests |
检测范围： | 7.8-500 pg/mL | 标记染料： | HRP |
仪器： | 比色酶标仪 | 储存： | -20°C |
操作时间： | 20 min | 蛋白名称： | IL-4 |
测试时间： | 3 hr 10 min | 特异性靶标位点： | |
一致性（未洗涤）： | No | 物种（经过测试）： | Human |
批间变异系数： | 7.1% | 检测试剂盒类型： | 即用型ELISA 试剂盒 |
批内变异系数： | 6.3% | 检测抗体偶联物： | Biotin |
| 同名基因： | BCGF-1, BCGF1, BSF-1, BSF1, IL-4 | 基因名称： | IL4 |
样品类型/体积： | 血清 50 µL 血浆 50µL 上清液 50µL 羊水 50µL 滑液 50µL 尿液 50µL | 组分： | - 预包被96孔板 - 生物素化检测抗体 - 链霉亲和素-辣根过氧化物酶 - 样品稀释液，冻干 - 标准曲线条 - 样品稀释液 - 洗涤浓缩液 - 底物溶液 - 终止液 - 裂解液10X - 胶板盖 |
| The Human Interleukin 4 (Hu IL-4) ELISA quantitates Hu IL-4 in human serum, plasma, cell culture supernatants, amniotic fluid, synovial fluid, urine or other body fluids. The assay will exclusively recognize both natural and recombinant Hu IL-4.人白介素4（Hu IL-4）ELISA定量了人血清，血浆，细胞培养上清液，羊水，滑液，尿液或其他体液中的Hu IL-4。 该检测试剂盒将只识别天然和重组的Hu IL-4。Principle of the method 方法原理The Human IL-4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人IL-4夹心ELISA（酶联免疫吸附测定）是测量匹配的抗体对之间结合的靶标量。 已在孔中预包被了靶标特异性抗体。 将样品或对照添加到这些孔中，并与固定的（捕获）抗体结合。 通过将第二（检测）抗体与捕获抗体不同表位上的靶标结合形成三明治。 与酶结合的抗体结合形成的三明治。 经过孵育和洗涤步骤以去除微孔板中未结合的物质后，添加底物溶液，该溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.白介素-4（IL-4）是由活化的T细胞产生的多效性免疫调节细胞因子，可作为白介素4受体的配体。 已经发现IL-4具有最广泛的作用范围。 白介素4受体也与IL13结合，这可能有助于该细胞因子和IL13的许多重叠功能。 STAT6是一种信号转导和转录激活因子，已显示在介导IL-4的免疫调节信号中起重要作用。 IL-4基因，IL-3，IL-5，IL-13和CSF2在5q染色体上形成细胞因子基因簇。 特别地，IL-4在染色体上紧邻IL13。 此外，发现IL-4基因，IL13和IL5在染色体上120Kb的范围内由几个远程调控元件协调。 IL-4δ2是IL-4的已知剪接变体。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
| 特点： |
| -极其简便快捷：操作时间仅15 min- 使用率更高：单独的标品板条，可检测96个样品- 适用客户：高度追求实验效率的客户 |
| 数据： |
IL-4 Human Instant ELISA™ KitRepresentative standard curve for Human IL-4 Instant ELISA |
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