Ig Isotyping Mouse Instant ELISA Kit/小鼠Ig同型鉴定一步法ELISA试剂盒
货号：88-50660-22
规格：2 x 96 tests
价格：6797
产品类型：Instant ELISA
品牌：Thermo Fisher
物种：小鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
产品规格： | 2 x 96 tests | 标记染料： | HRP |
仪器： | 比色酶标仪 | 储存： | -20°C |
操作时间： | 30 min | 蛋白名称： | Mouse Ig |
测试时间： | 2 hr 15 min | 特异性靶标位点： | |
一致性（未洗涤）： | No | 物种（经过测试）： | Mouse |
检测抗体偶联物： | Biotin | 检测试剂盒类型： | 即用型ELISA 试剂盒 |
样品类型/体积： | 上清液 10µL | 组分： | - 预包被96孔板 - 样品稀释液，冻干 - 小鼠阳性对照：小鼠IgG1，IgG2a，IgG2b，IgG2c，IgA，IgM，κ和λ； - 同型对照混合物（冻干） - 洗涤浓缩液20倍（磷酸盐缓冲液含1％Tween 20） - 底物溶液（四甲基联苯胺） - 终止液（1M磷酸） - 胶板盖 |
| The Mouse Immunoglobulin Isotyping ELISA quanlitatively detects Mouse Ig isotypes in mouse hybridoma cell culture supernatants.小鼠免疫球蛋白同种型ELISA定量检测小鼠杂交瘤细胞培养上清液中的小鼠Ig同种型。Principle of the method 方法原理Biotinylated monoclonal anti-mouse isotype specific or light chain specific capture antibodies bind to the Streptavidin coated plate. Ig Isotype present in the positive control or sample binds to the specific capture antibodies and is simultaneously bound by the HRP-conjugated detector antibody. Following incubation unbound reagents and sample components are removed during a wash step, and substrate solution reactive with HRP is added to the wells. A colored product is formed in proportion to the amount of soluble mouse Ig (H+L) present in the sample.生物素化的单克隆抗小鼠同种型特异性抗体或轻链特异性捕获抗体与链霉亲和素包被的板结合。 阳性对照或样品中存在的Ig同型与特异性捕获抗体结合，并同时与HRP结合的检测器抗体结合。 孵育后，在洗涤步骤中去除未结合的试剂和样品成分，并将与HRP反应的底物溶液添加到孔中。 与样品中存在的可溶性小鼠Ig（H + L）的数量成比例地形成有色产物。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.一抗的同种型及其应用可能导致背景染色。 一抗背景噪音可能是由于与靶细胞上的Fc受体结合而引起的。 通过与细胞蛋白，碳水化合物和脂质的非特异性相互作用； 或通过细胞自发荧光。 同型对照抗体可以充当阴性对照，以帮助将非特异性背景信号与特异性抗体信号区分开，因为它们对靶抗原没有相关特异性。 尽管同型对照最常用于流式细胞术，但它们可用于其他应用，例如染色质免疫沉淀（ChIP），免疫组织化学和凝胶迁移。 同型对照应与一抗种类和同种型匹配，以便可以准确确定一抗特异性染色的水平。 如果使用直接标记的第一抗体，则同型对照如果与测试抗体标记相同，则效果最佳。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
| 数据： |
Ig Isotyping Mouse Instant ELISA™ KitSuggested plate layout for Mouse Ig Isotyping Instant ELISA |
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