| The ProQuantum HumanIL-13Immunoassay Kit is designed to provide highly sensitive quantitative measurements of human IL-13 protein in small sample volumes. Utilizing proximity ligation assay (PLA) technology, the assay combines the analyte specificity of high-affinity antibody-antigen binding with the signal detection and amplification capabilities of real-time PCR to achieve a simple yet powerful next-generation protein quantitation platform. A user-friendly workflow combined with intuitive Cloud-based software for analytics enables sample-to-answer in just 2 hours.ProQuantum 免疫检测试剂盒进行少量人IL-13蛋白的高灵敏度定量检测。 利用邻近连接测定（PLA）技术，该测定将高亲和力抗体-抗原结合的分析物特异性与实时PCR的信号检测和扩增功能结合在一起，从而实现了一个简单而强大的下一代蛋白质定量平台。 友好的工作流程与直观的基于云的分析软件相结合，可在2小时内完成从样品到答案的分析。ProQuantum immunoassays utilize a matched pair of target-specific antibodies, each conjugated to a DNA oligonucleotide. During antibody-analyte binding, the two DNA oligos are brought into close proximity, which allows for ligation of the two strands and subsequent creation of a template strand for amplification. This platform leverages the sensitivity and large dynamic range of Applied Biosystems TaqMan real-time PCR technology.ProQuantum免疫测定法使用一对配对的靶标特异性抗体，每种抗体均与DNA寡核苷酸偶联。 在抗体-分析物结合过程中，两个DNA寡核苷酸紧密靠近，这允许两条链连接并随后创建模板链进行扩增。 该平台利用了Applied Biosystems TaqMan实时PCR技术的灵敏度和宽动态范围。The assay workflow is fast and easy—2 steps in 2 hours. There are a total of 7 components in each kit (Figure 2). First, mix the antibody-conjugates, dilute the sample, and create the standard curve in a working plate. Then, using a multi-channel pipette, add the antibody-conjugates and sample (or standard) into the wells of a PCR plate and incubate for 1 hour. Combine the master mix and ligase and add to the wells of the PCR plate, then run the plate on any qPCR instrument. After the run is complete, import the results file into the ProQuantum cloud-based software at https://apps.thermofisher.com/apps/proquantum. Using this software, the data can be analyzed easily to obtain protein concentration values. The software allows you to set up standard curves, design plate layouts, set up customized assay instructions, and obtain robust statistical group-wise comparisons.测定工作流程快速简便— 2小时内完成2个步骤。 每个试剂盒中总共有7个组件。 首先，混合抗体-结合物物，稀释样品，并在工作板上创建标准曲线。 然后，使用多通道移液器，将抗体结合物和样品（或标准品）添加到PCR板的孔中，并孵育1小时。 合并预混液和连接酶，并添加到PCR板的孔中，然后在任何qPCR仪器上运行该板。 运行完成后，将结果文件导入https://apps.thermofisher.com/apps/proquantum的基于ProQuantum云的软件中。 使用此软件，可以轻松分析数据以获得蛋白质浓度值。 该软件允许您设置标准曲线，设计板布局，设置定制化验说明并获得可靠的逐组统计比较。Target information 靶标信息IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.IL-13是主要由活化的Th2细胞产生的免疫调节细胞因子，参与调节炎症和免疫反应。 IL-13下调巨噬细胞活性，从而抑制促炎性细胞因子和趋化因子的产生，并通过独立于IgE和嗜酸性粒细胞的机制参与过敏原诱发的哮喘。 IL-13还调节B细胞成熟和分化的几个阶段，上调CD23和MHC II类表达，并促进B细胞的IgE同种型转换。 IL-13在结构上与IL-3，IL-4，IL-5和GM-CSF非常相似。 编码IL-13，IL3，IL5，IL4和CSF2的基因在5q染色体上形成细胞因子基因簇，其中IL-13基因特别接近IL4。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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