IL-6 Human ELISA Kit, High Sensitivity/人IL-6高灵敏ELISA试剂盒
货号：BMS213HS
规格：96 tests
价格：7353
产品类型：High-Sensitivity ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 0.03 pg/mL |
| 检测范围 | 0.08-5.0 pg/mL |
样本类型/体积 | 血浆 50 μL 血清 50 μL 上清液 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr |
| 批间变异系数 | 6% |
| 批内变异系数 | 4.9% |
Homogenous (no wash) | No |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 样品稀释液 - 分析缓冲浓缩液 - 生物素化检测抗体 - 放大稀释浓缩液 - 放大试剂Ⅰ - 放大试剂Ⅱ - SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | IL-6 |
| Protein aliases | B-cell differentiation factor, B-cell stimulatory factor 2, BSF-2, CDF, CTL differentiation factor, Hybridoma growth factor, IFN-beta-2, IL-6, Interferon beta-2, interleukin BSF-2, Interleukin-6 |
物种（Tested） | 人 |
试剂盒类型 | High Sensitivity Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene ID | (Human) 3569 |
| Gene aliases | BSF-2, BSF2, CDF, HGF, HSF, IFN-beta-2, IFNB2, IL-6, IL6 |
| Gene symbol | IL6 |
UniProt ID | (Human) P05231 |
| The Human High Sensitivity Interleukin 6 (HS IL-6) ELISA quantitates Hu HS IL-6 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu HS IL-6.人白介素6（HS IL-6）高灵敏度ELISA定量了人血清，血浆，缓冲液或细胞培养基中的人HS IL-6。 该检测试剂盒将专门识别天然和重组的人HS IL-6。Principle of the method 方法原理TheHuman HS IL-6solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A special amplification reagent step is added to achieve the high sensitivity capability. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人 HS IL-6固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。 已在孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 添加了特殊的扩增试剂步骤以实现高灵敏度。 经过孵育和洗涤步骤以去除微孔板中未结合的物质后，添加底物溶液，该溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。 Rigorous validation 严格验证 Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.白介素6（IL-6）是一种多功能的26 kD蛋白，最初是在RNA刺激的成纤维细胞样细胞的培养基中发现的。 IL-6似乎直接参与感染和细胞损伤后发生的反应，它可能被证明在调节急性期反应中作为IL-1和TNF-a很重要。据报道IL-6由成纤维细胞，活化的T细胞，活化的单核细胞或巨噬细胞和内皮细胞产生。 IL-6作用于多种细胞，包括骨髓祖细胞，T细胞，B细胞和肝细胞。另外，IL-6似乎在T淋巴细胞的增殖中与IL-2相互作用。 IL-6增强了IL-3对多能造血祖细胞的增殖作用。 IL-6在B细胞向浆细胞的分化中起关键作用，并且是浆细胞瘤和骨髓瘤的有效生长因子。 IL-6是一种非常有用的培养补充剂，可用于产生大量产生抗体的杂交瘤。 IL-6主要在急性和慢性炎症部位产生，被分泌到血清中并通过白介素6受体α诱导转录炎症反应。 IL-6的功能涉及多种与炎症相关的疾病状态，包括糖尿病和全身性青少年类风湿性关节炎。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
| 数据： |
| IL-6 Human ELISA Kit, High SensitivityRepresentative standard curve for Human IL-16 High Sensitivity ELISA |
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