ICAM-1 Human ELISA Kit, Extra Sensitive/人ICAM-1超灵敏ELISA试剂盒
货号：BMS241,BMS241TEN
规格：96 Tests,10X96 Tests
价格：6120,35475
产品类型：High-Sensitivity ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 0.33 ng/mL |
| 检测范围 | 0.63-10.0 ng/mL |
样本类型/体积 | 血浆 10 μL 血清 10 μL 上清液 100 μL 尿液 100 μL 胆汁 100 μL 羊水 100μL |
操作时间 | 1 hr 20 min |
测试时间 | 2 hr 10 min |
| 批间变异系数 | 7.8% |
| 批内变异系数 | 5.6% |
Homogenous (no wash) | No |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 10X96 Tests |
组份 | - 预包被96孔板 - 标准品 - 样品稀释液 - 分析缓冲浓缩液 - HRP-偶联的检测抗体 - 洗涤液 - 底物 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | ICAM-1 (CD54) |
| Protein aliases | CD54, cell surface glycoprotein P3.58, ICAM-1, Major group rhinovirus receptor, Intercellular adhesion molecule 1, intercellular adhesion molecule 1 (CD54), human rhinovirus receptor |
物种（Tested） | 人 |
试剂盒类型 | High Sensitivity Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | HRP |
| Gene ID | (Human) 3383 |
| Gene aliases | BB2, CD54, ICAM1, P3.58 |
| Gene symbol | ICAM1 |
UniProt ID | (Human) P05362 |
| The Human Intercellular Adhesion Molecule-1 (Hu ICAM-1) ELISA quantitates Hu ICAM-1 in human serum, plasma, amniotic fluid, spontaneous urine. bile, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu ICAM-1.人类细胞间粘附分子1（Hu ICAM-1）ELISA定量分析了人类血清，血浆，羊水，自发尿液中的Hu ICAM-1。 胆汁或细胞培养基。 该检测试剂盒将专门识别天然和重组Hu ICAM-1。Principle of the method 方法原理The Human ICAM-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人 ICAM-1固相夹心ELISA（酶联免疫吸附测定）旨在测量匹配的抗体对之间结合的靶标量。 已在孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。 Rigorous validation 严格验证 Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.ICAM-1（CD54）是一种85-110 kDa单链1型整合膜糖蛋白，具有五个免疫球蛋白超家族重复序列，一个跨膜区和一个胞质区的胞外域。 ICAM-1具有7个潜在的N-连接的糖基化位点，并且与ICAM-3（CD50）和ICAM-2（CD102）具有相当的氨基酸序列同源性。 ICAM-1与CD11a / CD18（白细胞粘附分子，LFA-1）或CD11b / CD18（Mac-1）类型的整合素结合，并被鼻病毒用作受体。 ICAM-1由活化的内皮细胞表达，并在上皮细胞，成纤维细胞，软骨细胞，B淋巴细胞，T淋巴细胞（低），单核细胞，巨噬细胞，树突状细胞和嗜中性粒细胞中检测到，其水平随着炎症而增加。 在某些癌细胞和黑色素瘤细胞中也检测到ICAM-1。 在血浆中可检测到可溶性ICAM-1，在患有各种炎症综合症的患者中可溶性ICAM-1升高。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
| 数据： |
| ICAM-1 Human ELISA Kit, Extra SensitiveRepresentative standard curve for Human ICAM-1 ELISA |
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