BHQ-2 CPG, Glycolate, Super Column; 500 Å
货号：SCG5-5042G-1,SCG5-5042G-2,SCG5-5042G-5
规格：1 µmol,200 nmol,50 nmol
价格：1055.25,281.4,196.98
产品类型：核酸合成
品牌：LGC Biosearch Technologies
| A BHQ®-2 Glycolate Super Column is packed with BHQ-2 glycolate CPG 500 Å and is for use on high-throughput DNA synthesizers. BHQ-2 CPG is used to add the non-fluorescent quencher to the 3' end of an oligonucleotide. BHQ-2 quencher has maximum absorption in the 540-670 nm range, which provides excellent quenching of fluorophores emitting in this range, eg fluorescein, TAMRA, ROX®, Texas Red® and Cy™3. BHQ-2 Glycolate CPG has a glycolate linkage to the CPG support. The glycolate linker allows for rapid cleavage of the oligonucleotides and is labile enough for base sensitive oligonucleotides. The 500 Å CPG is useful for the synthesis of oligos up to 50-mers in length, especially when larger amounts of product are desired, as it allows for greater nucleoside loading amounts than do supports with larger pore sizes. |
| 特性： |
| ▪ Appearance:purple powder▪ Absorption Maximum (Lambda Max):579▪ Extinction Coefficient at Lambda max:38000▪ Extinction Coefficient at 260 nm:8000 |
| 产品用途： |
| Synthesis conditions:Follow the instrument manufacturer's protocol, with noted changes, when using this product.Cleavage conditions:When using fast deprotecting amidites use concentrated Ammonia for 45 min at 25 °C or Ammonia/ Methylamine (AMA) (1:1) for 15 min at 25 °C; or
t-butylamine/H2O (1:3) for 90 min at 25 °C (TAMRA requires deprotection with TBA). When using standard amidites (eg. C-Bz, G-iBu) use concentrated Ammonia for 45 min at 25 °C.Deprotection conditions:When using fast deprotecting amidites (eg. C-Ac, G-DMF, G-PAC) deprotect in concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) deprotect in concentrated ammonia for 5 hours at 60 °C. If you are using base sensitive fluorophores (ie. TAMRA) we recommend deprotecting the oligonucleotide in t-butylamine/H2O(1:3) for 12-16 hours at 60 °C.ABI 3900 users: Columns should be compatible with standard ABI protocols. However, when using 50 nmol Super Columns, the following changes are recommended:
1) Change the head pressure/purge pressure/chamber pressure to 3.5 psi.
2) An additional oxidation step
3) OPTIONAL (but may help): extension of the coupling time to 20 sec Image of cleaved and deprotected structure: The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is：556.46 |
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