Th1/Th2 Mouse Uncoated ELISA Kit/小鼠Th1/Th2 未包被ELISA试剂盒

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Th1/Th2 Mouse Uncoated ELISA Kit/小鼠Th1/Th2 未包被ELISA试剂盒

货号:88-7711-44

规格:96 tests

价格:6537

产品类型:自定义ELISA

品牌:Thermo Fisher

物种:小鼠

宿主:其它

抗体亚型:其它

荧光染料:其它

样本类型/体积

血清 100 μL

血浆 100 μL

上清液 100 μL

操作时间

1 hr 45 min

测试时间

24 hr 30 min

Homogenous (no wash)

No

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

96 Tests

组份

- 捕获抗体:Pre-titrated, 纯化抗体

- 检测抗体:Pre-titrated, Biotin偶联抗体

- 标准品:重组细胞因子,用于生成标准曲线和校准样品

-ELISA/ELISPOT包被缓冲液粉末

- 检测稀释液:5X浓缩液

- 检测酶:Pre-titrated Streptavidin-HRP

- 底物溶液:Tetramethylbenzidine (TMB) 底物溶液

- 分析证书:特定批次说明书用于抗体和标准品稀释

储存温度

2-8℃

Protein nameTh1/Th2
Protein aliasesB-cell growth factor 1, B-cell IgG differentiation factor, B-cell stimulatory factor 1, BSF-1, CSIF, Cytokine synthesis inhibitory factor, gamma interferon, IFN-gamma, IGG1 induction factor, IL-10, IL-2, IL-4, Interferon gamma, Interleukin-10, Interleukin-2, Interleukin-4, Lymphocyte stimulatory factor 1, T-cell growth factor, TCGF

物种(Tested)

小鼠

试剂盒类型

Uncoated ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesBSF-1, CSIF, Ifg, IFN-g, Ifng, Il-10, Il-2, Il-4, Il10, Il2, Il4
Gene ID(Mouse) 15978,(Mouse) 16153,(Mouse) 16183,(Mouse) 16189
Gene symbolIfng, Il10, Il2, Il4
UniProt ID(Mouse) P01580,(Mouse) P18893,(Mouse) P04351,(Mouse) P07750
The Mouse Th1/Th2 Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse Th1/Th2 proteins (Interferon gamma, IL-2, IL-4 and IL-10). Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.小鼠 Th1 / Th2 未包被 ELISA 试剂盒包含预先匹配的抗体对,板和试剂,用于进行定量酶联免疫吸附测定(ELISA),以检测和定量小鼠Th1 / Th2蛋白(干扰素gamma,IL-2,IL- 4和IL-10)。 需要洗涤液和终止液才能完成ELISA反应,需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA是测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被到微孔板孔的底部,这是一整夜的过程。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息T helper type 1 (Th1) cells and T helper type 2 (Th2) cells are both a lineage of CD4 effector T cells. Th1 and Th2 are involved in promoting cell-mediated immunity. Th1 cells secrete IFN-gamma, IL-2, IL-10, and TNF-alpha/beta, which are required for host defense against intracellular viral and bacterial pathogens. Th2 cells secrete IL-4, IL-5, IL-9, IL-13, and IL-17E/IL-15, which are required for humoral immunity and play an important role in coordinating the immune response to larger extracellular pathogens.T辅助1型(Th1)细胞和T辅助2型(Th2)细胞都是CD4效应T细胞的谱系。 Th1和Th2参与促进细胞介导的免疫。 Th1细胞分泌IFN-γ,IL-2,IL-10和TNF-α/β,这是宿主防御细胞内病毒和细菌病原体所必需的。 Th2细胞分泌IL-4,IL-5,IL-9,IL-13和IL-17E / IL-15,这是体液免疫所必需的,并且在协调对更大的细胞外病原体的免疫应答中起重要作用。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
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