Th1/Th2 Mouse Uncoated ELISA Kit/小鼠Th1/Th2 未包被ELISA试剂盒
货号:88-7711-44
规格:96 tests
价格:6537
产品类型:自定义ELISA
品牌:Thermo Fisher
物种:小鼠
宿主:其它
抗体亚型:其它
荧光染料:其它
样本类型/体积 | 血清 100 μL 血浆 100 μL 上清液 100 μL |
操作时间 | 1 hr 45 min |
测试时间 | 24 hr 30 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 捕获抗体:Pre-titrated, 纯化抗体 - 检测抗体:Pre-titrated, Biotin偶联抗体 - 标准品:重组细胞因子,用于生成标准曲线和校准样品 -ELISA/ELISPOT包被缓冲液粉末 - 检测稀释液:5X浓缩液 - 检测酶:Pre-titrated Streptavidin-HRP - 底物溶液:Tetramethylbenzidine (TMB) 底物溶液 - 分析证书:特定批次说明书用于抗体和标准品稀释 |
储存温度 | 2-8℃ |
Protein name | Th1/Th2 |
Protein aliases | B-cell growth factor 1, B-cell IgG differentiation factor, B-cell stimulatory factor 1, BSF-1, CSIF, Cytokine synthesis inhibitory factor, gamma interferon, IFN-gamma, IGG1 induction factor, IL-10, IL-2, IL-4, Interferon gamma, Interleukin-10, Interleukin-2, Interleukin-4, Lymphocyte stimulatory factor 1, T-cell growth factor, TCGF |
物种(Tested) | 小鼠 |
试剂盒类型 | Uncoated ELISA Kit |
标记(染料) | HRP |
检测抗体偶联物 | Biotin |
Gene aliases | BSF-1, CSIF, Ifg, IFN-g, Ifng, Il-10, Il-2, Il-4, Il10, Il2, Il4 |
Gene ID | (Mouse) 15978,(Mouse) 16153,(Mouse) 16183,(Mouse) 16189 |
Gene symbol | Ifng, Il10, Il2, Il4 |
UniProt ID | (Mouse) P01580,(Mouse) P18893,(Mouse) P04351,(Mouse) P07750 |
The Mouse Th1/Th2 Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse Th1/Th2 proteins (Interferon gamma, IL-2, IL-4 and IL-10). Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.小鼠 Th1 / Th2 未包被 ELISA 试剂盒包含预先匹配的抗体对,板和试剂,用于进行定量酶联免疫吸附测定(ELISA),以检测和定量小鼠Th1 / Th2蛋白(干扰素gamma,IL-2,IL- 4和IL-10)。 需要洗涤液和终止液才能完成ELISA反应,需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA是测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被到微孔板孔的底部,这是一整夜的过程。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息T helper type 1 (Th1) cells and T helper type 2 (Th2) cells are both a lineage of CD4 effector T cells. Th1 and Th2 are involved in promoting cell-mediated immunity. Th1 cells secrete IFN-gamma, IL-2, IL-10, and TNF-alpha/beta, which are required for host defense against intracellular viral and bacterial pathogens. Th2 cells secrete IL-4, IL-5, IL-9, IL-13, and IL-17E/IL-15, which are required for humoral immunity and play an important role in coordinating the immune response to larger extracellular pathogens.T辅助1型(Th1)细胞和T辅助2型(Th2)细胞都是CD4效应T细胞的谱系。 Th1和Th2参与促进细胞介导的免疫。 Th1细胞分泌IFN-γ,IL-2,IL-10和TNF-α/β,这是宿主防御细胞内病毒和细菌病原体所必需的。 Th2细胞分泌IL-4,IL-5,IL-9,IL-13和IL-17E / IL-15,这是体液免疫所必需的,并且在协调对更大的细胞外病原体的免疫应答中起重要作用。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
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