Mouse anti-Rat IgG2c Secondary Antibody, PE, eBioscience™/小鼠抗大鼠IgG2c 二抗, PE, eBioscience™

货号：12-4816-82

规格：100 µg

价格：1556

产品类型：荧光二抗

品牌：eBioscience

物种：大鼠

宿主：小鼠

抗体亚型：其它

克隆号：R2C-23A3

荧光染料：PE

类型:	二抗	同型对照：	IgG1
浓度：	0.2mg/mL	用法：	0.06 µg/test(tests)

产品详细信息Description: The monoclonal antibody R2C-23A3 recognizes Rat IgG2c antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other rat or mouse antibody isotypes.Applications Reported: This R2C-23A3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This R2C-23A3 antibody has been tested by flow cytometric analysis of cell stained with rat IgG2c primary antibody. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

Rat IgG2c Secondary Antibody (12-4816-82) in FlowStaining of C57Bl/6 splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and buffer (left) or Anti-Mouse CD24 Purified (clone 30-F1, a rat IgG2c isotype) (Product # 14-0241) (middle) and subsequently stained 0.03 µg of Anti-Rat IgG2c PE. Right: Comparison to direct staining with Anti-Mouse CD3e FITC (Product # 11-0031-82) and Anti-Mouse CD24 PE (Product # 12-0241-82). Total viable cells were used for analysis.

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