IL-17F (Homodimer) Human Uncoated ELISA Kit with Plates/人IL-17F (Homodimer) ELISA试剂盒含未包被板

货号：88-7478-22,88-7478-86

规格：2X96 Tests,10X96 Tests

价格：3125,6537

产品类型：自定义ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	16 pg/mL
检测范围	16-2,000 pg/mL
样本类型/体积	血清 100 μL 血浆 100 μL 上清液 100 μL
操作时间	1 hr 45 min
测试时间	24 hr 30 min
Homogenous (no wash)	No
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10X 96 Tests
组份	- 捕获抗体：Pre-titrated, 纯化抗体 - 检测抗体：Pre-titrated, Biotin偶联抗体 - 标准品：重组细胞因子，用于生成标准曲线和校准样品 -包被缓冲液：10X Carbonate ELISA包被缓冲液 - 检测稀释液：5X浓缩液 - 检测酶：Pre-titrated Avidin-HRP - 底物溶液：Tetramethylbenzidine (TMB)溶液 - 分析证书：特定批次说明书用于抗体和标准品稀释 - 96孔板：NUNC Maxisorp flat-bottom（含产品目录号，后缀 -22，-44,-76，-86）
储存温度	2-8℃
Protein name	IL-17F
Protein aliases	Cytokine ML-1, IL-17F, Interleukin-17F
物种（Tested）	人
试剂盒类型	Uncoated ELISA Kitwith Plates
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	CANDF6, IL-17F, IL17F, ML-1, ML1
Gene ID	(Human) 112744
Gene symbol	IL17F
UniProt ID	(Human) Q96PD4

The Human Interleukin 17F (Homodimer) (IL-17F) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-17F. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.人白介素17F（同型二聚体）（IL-17F）未包被的ELISA试剂盒包含预匹配的抗体对，板和试剂，用于进行定量酶联免疫吸附测定（ELISA），以检测和定量人IL-17F的蛋白质水平。 需要洗涤液和终止液才能完成ELISA反应，需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA是测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被到微孔板孔的底部，这是一整夜的过程。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息IL-17F (Interleukin 17F, CTLA-8)) is a cytokine belonging to the IL-17 family that is produced by inflammatory cells such as activated T cells, mast cells, and basophils. IL-17F is involved in allergic airway inflammation, and can induce several cytokines, chemokines, and adhesion molecules in bronchial epithelial cells, vein endothelial cells, fibroblasts, and eosinophils. IL-17F may be secreted as a homodimer, or a heterodimer with IL17A. It acts by binding to the type I receptor, IL-17R, aiding recruitment of monocytes and neutrophils at the site of inflammation by increasing chemokine production. IL-17F also stimulates induction of other pro-inflammatory cytokines TNF alpha, IL-1 beta, IL-6, and IL-8, and reports strongly suggest the involvement of IL-17 in several chronic inflammatory diseases such as rheumatoid arthritis, psoriasis and multiple sclerosis. TGF-beta (differentiation) and IL-23 (expansion) are required for induction and maintenance of Th17 (IL-17 producing) cells, which in turn induce the other pro-inflammatory cytokines. IL-17F is produced, and exists, as a homo-dimer, with homology to a herpes virus early protein, is one of the six members (IL-17A-F) of this cytokine family, and is well characterized and highly expressed by activated effector memory T cells. IL-17F has been found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1.IL-17F（白介素17F，CTLA-8）是属于IL-17家族的一种细胞因子，是由诸如活化T细胞，肥大细胞和嗜碱性粒细胞之类的炎性细胞产生的。 IL-17F与过敏性气道炎症有关，可在支气管上皮细胞，静脉内皮细胞，成纤维细胞和嗜酸性粒细胞中诱导多种细胞因子，趋化因子和粘附分子。 IL-17F可能被分泌为IL17A的同二聚体或异二聚体。它通过与I型受体IL-17R结合而起作用，通过增加趋化因子的产生来帮助在炎症部位募集单核细胞和中性粒细胞。 IL-17F还刺激其他促炎性细胞因子TNFα，IL-1 beta，IL-6和IL-8的诱导，并且有报告强烈表明IL-17参与了几种慢性炎症性疾病，如类风湿性关节炎，牛皮癣和多发性硬化症。 TGF-beta（分化）和IL-23（扩增）是诱导和维持Th17（产生IL-17的细胞）所必需的，后者又会诱导其他促炎性细胞因子。 IL-17F产生并以与疱疹病毒早期蛋白同源的同型二聚体形式存在，是该细胞因子家族的六种成员之一（IL-17A-F），并具有良好的特征和表达能力激活的效应记忆T细胞。已经发现IL-17F抑制内皮细胞的血管生成并诱导内皮细胞产生IL2，TGFB1 / TGFB和单核细胞趋化蛋白-1。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

技术参数

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-21229.htm