Rabbit anti-Human IgE Recombinant Secondary Antibody, Biotin/兔抗人IgE重组二抗，Biotin

货号：MA5-27927

规格：50 µg

价格：3994

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Human IgE

物种：人

宿主：兔

抗体亚型：IgE

克隆号：RM122

荧光染料：Biotin

类型:	二抗	同型对照：
浓度：	1 mg/mL	用法：	0.5 µg/mL-2 µg/mL(ICC)；0.01 µg/mL-0.1 µg/mL(ELISA)；0.5 µg/mL-2 µg/mL(IHC)

产品详细信息Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.靶标信息Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Human IgE Secondary Antibody (MA5-27927) in ELISAELISA analysis of Human IgE with human IgE lambda from human myeloma plasma and IgE from human plasma at a concentration of 50 ng/well. Sample was incubated with Human IgE monoclonal antibody (Product # MA5-27927) at a dilution of 200 ng/mL, 50 ng/mL, and 10 ng/mL, followed by alkaline phosphatase conjugated anti-rabbit IgG secondary antibody. There was no cross reaction to human IgG, IgM, IgD, or IgA. Human IgE Secondary Antibody (MA5-27927) in ELISAELISA analysis of Human IgE with human IgE. Sample was incubated with Human IgE monoclonal antibody (Product # MA5-27927) using a serial dilution, followed by alkaline phosphatase conjugated anti-rabbit IgG secondary antibody.

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