Rabbit anti-Human IgG1 Recombinant Secondary Antibody/兔抗人IgG1重组二抗
货号：SA5-10202
规格：100 µg
价格：2732
产品类型：二抗
品牌：Thermo Fisher
抗原：Peptide corresponding to the hinge region of Human
物种：人
宿主：兔
抗体亚型：IgG
荧光染料：其它
| 抗体类型: | 二抗 | 同型对照： | |
| 浓度： | 1 mg/mL | 用法： | 50-200 ng/well (Capture), 0.05-0.2 µg/mL (Detect)(ELISA)；0.5-2 µg/mL(ICC)； 0.5-2 µg/mL(IF)；0.5-2 µg/mL(IHC) |
产品详细信息This antibody reacts to the heavy chain of human IgG1. No cross reactivity with human IgG2, IgG3, IgG4, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.靶标信息IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.
| 数据： |
Human IgG1 Secondary Antibody (SA5-10202) in IHC (P)Immunohistochemical staining of FFPE Tonsil tissue sections using Anti-Human IgG1 antibody (Product # SA5-10202) at a 1:15,000 dilution. Human IgG1 Secondary Antibody (SA5-10202) in ELISAELISA of human immunoglobulins shows Rabbit Anti-Human IgG1 Secondary Antibody (Product # SA5-10202) only reacts to human IgG1. No cross reactivity with Human IgG2, IgG3, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of Rabbit Anti-Human IgG1 Secondary Antibody (Product # SA5-10202) was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. Human IgG1 Secondary Antibody (SA5-10202) in ELISA A titer ELISA using Rabbit Anti-Human IgG1 Secondary Antibody (Product # SA5-10202). The plate was coated with different amounts of human IgG1. A serial dilution of Rabbit Anti-Human IgG1 Secondary Antibody (Product # SA5-10202) was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. |
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技术参数 产品应用 ELISA;ICC;IF;IHC
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