Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-Cyanine5.5, eBioscience™/小鼠IgG1 kappa同型对照 (P3.6.2.8.1), PerCP-Cyanine5.5, eBioscience™

货号： 45-4714-82 , 45-4714-80

规格：100 µg,25 µg

价格：2448,934

产品类型：小鼠同型对照

品牌：eBioscience

物种：小鼠

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：P3.6.2.8.1

荧光染料：PerCP-Cy5.5

类型:	同型对照	同型对照：
浓度：	0.2 mg/mL	用法：	Assay-Dependent(Ctrl);Assay-Dependent(Flow)

产品详细信息Description: The monoclonal mouse IgG1 K immunoglobulin is useful as an isotype control.Applications Reported: The PerCP-Cy5.5 Mouse IgG1 K Isotype Control has been reported for use in flow cytometric analysis.Applications Tested: The Mouse IgG1 K Isotype Control has been tested by flow cytometric analysis. Use isotype control at the same concentration as experimental antibody.Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

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参考文献：

1.Nature communicationsIL-10 inhibits neuraminidase-activated TGF-β and facilitates Th1 phenotype during early phase of infection.2.PloS oneSoluble tumor necrosis factor related apoptosis inducing ligand level as a predictor of severity of sepsis and the risk of mortality in septic patients.3.Veterinary researchImmunobiotic lactic acid bacteria beneficially regulate immune response triggered by poly(I:C) in porcine intestinal epithelial cells.

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