Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, FITC/山羊抗小鼠IgG (H+L)交叉吸附二抗

货号：F-2761

规格：1 mg

价格：2701

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Gamma Immunoglobins Heavy and Light chains

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：FITC

点击查看所有Alexa Fluor 荧光二抗

抗体类型:	荧光二抗	同型对照：
浓度：	2mg/mL	用法：	1-10 µg/mL(Flow);1-10 µg/mL（ICC);1-10 µg/mL(IF);1-10 µg/mL(IHC);Assay Dependent(IHC(F))

靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (F-2761) in IFA 20-second time series showing enhanced resistance to photobleaching afforded by ProLong® Gold antifade reagent. Fixed bovine pulmonary artery endothelial cells were labeled with anti–α-tubulin (Product # A11126) and visualized with fluorescein goat anti-mouse IgG (Product # F-2761). The samples were mounted in ProLong® Gold antifade reagent (Product # P36930; top) or phosphate-buffered saline (bottom). Images were acquired at 5 second intervals using a 40x/1.3 NA oil immersion objective with continuous illumination from a standard 100 watt Hg-arc lamp. Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (F-2761) in IFThe relative photobleaching rates of fluorescein and Texas Red® fluorophores with buffer alone (right series) or after treatment with the ProLong® antifade reagent (left series). Bovine pulmonary artery endothelial cells were fixed, permeabilized and labeled with Texas Red®-X phalloidin (Product # T7471), which labels F-actin, and with a mouse monoclonal anti-ß-tubulin antibody and fluorescein goat anti-mouse IgG antibody (Product # F-2761), which label microtubules. Images were acquired at appropriate wavelengths using a cooled CCD camera. Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (F-2761) in IF Increased label specificity and resolution afforded by Image-iT® FX signal enhancer. Fixed and permeabilized MRC-5 human lung fibroblast cells were labeled with mouse anti-human Golgin-97 primary antibody (Product # A-21270), then visualized with either fluorescein goat anti-mouse IgG (F2761, top row) or Alexa Fluor® 488 Goat Anti-Mouse IgG (Product # A-11001, bottom row). Cells on the right were treated with Image-iT® FX signal enhancer (Product # I36933) prior to antibody incubation. (Product # A-21270).

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参考文献：

1.Stem cells internationalAdipose-Derived Stem Cells Cocultured with Chondrocytes Promote the Proliferation of Chondrocytes.2.Clinical and experimental immunologyProgrammed death-1 ligands 1 and 2 expression in cutaneous squamous cell carcinoma and their relationship with tumour- infiltrating dendritic cells.3.Diseases of aquatic organismsPathogenesis of a Thai strain of white spot syndrome virus (WSSV) in juvenile, specific pathogen-free Litopenaeus vannamei.4.OncotargetThe ISG15-specific protease USP18 regulates stability of PTEN.5.Journal of fish diseasesKinetic analysis of internalization of white spot syndrome virus by haemocyte subpopulations of penaeid shrimp, Litopenaeus vannamei (Boone), and the outcome for virus and cell.

技术参数

产品应用 Flow;ICC;IHC;IHC(F);IF;WB

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