Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP/山羊抗小鼠IgG (H+L)二抗，HRP

货号：62-6520

规格：1 mL

价格：2373

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Gamma Immunoglobins Heavy and Light chains

物种：小鼠

宿主：重组抗体

抗体亚型：IgG

荧光染料：HRP

抗体类型:	荧光二抗	同型对照：
浓度：	1.5 mg/mL	用法：	1:2,000-1:4,000（ELISA）； 1:2,000-1:4,000 (IHC)； 1:2,000-1:10,000 (WB)

产品详细信息

ZyMAX antibodies are specifically isolated from antigen-affinity columns using advanced elution protocols, leaving only the highest affinity, antigen-specific antibodies. ZyMAX conjugates are prepared with modified cross-linkers to achieve optimal conjugation ratios and stability. Improved purification methods virtually eliminate unconjugated components, giving superior sensitivity and lowest possible levels of background.靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG (H+L) Secondary Antibody (62-6520) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of Caco-2 (Lane 1), NTERA-2 (Lane 2), THP1 (Lane 3), HEL 92.1.7 (Lane 4), HEL 92.1.7 treated with 1 uM Doxorubicin for 18 hours (Lane 5), HeLa (Lane 6), and HeLa treated with 1 uM Doxorubicin for 18 hours (Lane 7). The blots were probed with Anti-Cyclin A Mouse Monoclonal Antibody (Product # MA5-11306, 1:100-1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 62-6520, 1:4000 dilution). A ~ 52 kDa band corresponding to Cyclin A was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106). Mouse IgG (H+L) Secondary Antibody (62-6520) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of T47D (Lane 1), A549 (Lane 2), U2OS (Lane 3), K562 (Lane 4), HeLa (Lane 5), Raji (lane 6), HEK-MSR (lane 7), MCF7 (lane 8), MDA-MB-231 (lane 9), LNCaP (lane 10) and Hep G2 (lane 11). The blots were probed with Anti-PRMT3 Mouse Monoclonal Antibody (Product # 730020, 0.5-1 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 62-6520, 1:4000 dilution). A 59 kDa band corresponding to PRMT3 was observed across cell lines tested expect Raji and HEK-MSR. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1. Frontiers in physiologyThe Apical Localization of Na+, K+-ATPase in Cultured Human Retinal Pigment Epithelial Cells Depends on Expression of the β2 Subunit.2. Scientific reportsFocal adhesions control cleavage furrow shape and spindle tilt during mitosis.3. PloS oneImmunological and Functional Characterization of RhoGDI3 and Its Molecular Targets RhoG and RhoB in Human Pancreatic Cancerous and Normal Cells.4. Journal of neuroinflammationThe involvement of mast cells in the irinotecan-induced enteric neurons loss and reactive gliosis.5. Cell death and diseaseInduction of intestinal stemness and tumorigenicity by aberrant internalization of commensal non-pathogenic E. coli.

技术参数

产品应用 ELISA;ICC;IHC;WB;IHC(P)

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-21620.htm