Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP/山羊抗小鼠IgG (H+L)交叉吸附二抗，HRP

货号：31432

规格：1.5 mL

价格：2453

产品类型：荧光二抗

品牌：Thermo Fisher

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：HRP

抗体类型:	荧光二抗	同型对照：
浓度：	0.8 mg/mL	用法：	1:10,000-1:100,000 (WB)； 1:500-1:5,000 (ICC); 1:500-1:5,000 (IHC)

产品详细信息Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.Product # 31432 has been successfully used in Western blot, and ICC applications.Antibody Specificity: This antibody reacts with whole molecule of mouse IgG and with the light chains of other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine, and horse serum proteins. However, this antibody may cross-react with immunoglobulins from other species.Restoration and Storage: Store product at 4°C until opened. Restore with 1.5 mL distilled water (0.8 mg/mL after restoration). Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. To judge clarity, draw product into a pasteur pipette. Product may be stored for several weeks at 4°C as an undiluted liquid. After dilution, do not use for more than one day.To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.Country of Origin: USA靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据：

Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (31432) in WB Western blot analysis was performed on whole cell extracts (30 µg lysate) of K-562 (Lane 1) and U-87 MG (Lane 2). The blots were probed with Anti-SOD2 Mouse Monoclonal Antibody (Product # MA1-106, 2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP conjugate (Product # 31432) at dilutions 1:10,000 (Fig. 1) and 1:100,000 (Fig. 2). A 25 kDa band corresponding to SOD2 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE®12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

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参考文献：

1. The Journal of biological chemistryAutophosphorylation-based Calcium (Ca2+) Sensitivity Priming and Ca2+/Calmodulin Inhibition of Arabidopsis thaliana Ca2+-dependent Protein Kinase 28 (CPK28).2. The journal of histochemistry and cytochemistry : official journal of the Histochemistry SocietySialidase Unmasks Mucin Domain Epitopes of Lubricin.3. The Annals of thoracic surgeryRegional Disruptions in Endothelial Nitric Oxide Pathway Associated With Bicuspid Aortic Valve.4. Journal of virologyAltered Glycosylation Patterns Increase Immunogenicity of a Subunit Hepatitis C Virus Vaccine, Inducing Neutralizing Antibodies Which Confer Protection in Mice.5. The ProstateAndrogen deprivation induces senescence characteristics in prostate cancer cells in vitro and in vivo.

技术参数

产品应用 ICC;IHC;WB;ELISA

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