Rabbit anti-Mouse IgG2a Secondary Antibody, HRP/兔抗小鼠IgG2a二抗，HRP

货号：61-0220

规格：1 mL

价格：1964

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Mouse IgG2a

物种：小鼠

宿主：兔

抗体亚型：IgG

荧光染料：HRP

类型:	二抗	同型对照：
浓度：	1 mg/mL	用法：	1:1,000-1:2,000(ELISA)；1:1,000-1:10,000(WB)

产品详细信息This monoclonal Rabbit anti-Mouse IgG2a reacts with mouse IgG2a and exhibits no reactivity to the other mouse IgG subclasses, IgA, IgM or light chains.靶标信息IgM (Immunoglobulin M) is expressed intracellularly during the early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. The isotype of a primary antibody and its application can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are also useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

数据

Mouse IgG2a Secondary Antibody (61-0220) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of K-562 (Lane 1) and U-2 OS (Lane 2). The blots were probed with Anti LKB1 Mouse Monoclonal Antibody (Product # AHO1392, 2 µg/mL) and detected by chemiluminescence using Rabbit anti-Mouse IgG (H+L) Secondary Antibody, HRP (Product # 61-0220) at dilutions 1:1,000 (Fig. 1), 1:5,000 (Fig. 2) 1:10,000 and (Fig. 3). A 55 kDa band corresponding to LKB1 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), , XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106)

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参考文献：

1.Journal of biomedical science Characterization of cross protection of Swine-Origin Influenza Virus (S-OIV) H1N1 and reassortant H5N1 influenza vaccine in BALB/c mice given a single-dose vaccination. 2.Journal of immunology IL-13 is a key regulatory cytokine for Th2 cell-mediated pulmonary granuloma formation and IgE responses induced by Schistosoma mansoni eggs.

技术参数

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