Rat anti-Mouse IgG1 Secondary Antibody, eFluor 660，eBioscience/大鼠抗小鼠lgG1二抗，eFluor 660

货号：50-4015-82,50-4015-80

规格：100 µg,25 µg

价格：2651,1023

产品类型：荧光二抗

品牌：eBioscience

物种：小鼠

宿主：大鼠

抗体亚型：IgG

克隆号：M1-14D12

荧光染料：eFluor 660

类型:	二抗	同型对照：
浓度：	0.2 mg/mL	用法：	0.25 µg/test(Flow)；10 µg/mL(ICC)；10 µg/mL(IF)；10 µg/mL(IHC)

产品详细信息Description: The monoclonal antibody M1-14D12 recognizes Mouse IgG1 antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG1 or any rat isotype antibodies.Applications Reported: This M1-14D12 antibody has been reported for use in flow cytometric analysis, microscopy, immunohistochemical staining, and immunocytochemistry.Applications Tested: This M1-14D12 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells stained first with a primary antibody of the mouse IgG1 isotype. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µg. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This M1-14D12 antibody has also been tested by immunocytochemistry on fixed and permeabilized cells that have been stained first with a primary antibody of the mouse IgG1 isotype. For immunocytochemistry and immunohistochemistry, this can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondaryantibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG1 Secondary Antibody (50-4015-82) in ICCImmunocytochemistry of fixed and permeabilized HeLa cells using 1 µg/mL of Anti-alpha Tubulin Purified (Product # 14-4502-82), followed by 10 µg/mL of Anti-Mouse IgG1 eFluor® 660. Nuclei are stained with DAPI. Mouse IgG1 Secondary Antibody (50-4015-82) in FlowStaining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control Purified (blue histogram) or Anti-Human CD4 Purified (RPA-T4, a mouse IgG1 antibody) (Product # 14-0049-82) (purple histogram) followed by 0.25 µg of Anti-Mouse IgG1 eFluor® 660. Cells in the lymphocyte gate were used for analysis.

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