Donkey anti-Mouse IgG (H+L) Secondary Antibody, WesternDot 625/驴抗小鼠IgG（H + L）二抗，WesternDot 625

货号：W10805

规格：25 blots

价格：0

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Mouse Immunoglobins

物种：小鼠

宿主：驴

抗体亚型：IgG

荧光染料：WesternDot 625

类型:	二抗	同型对照：
浓度：		用法：	1:500(WB)

产品详细信息WesternDot fluorescently labeled secondary antibodies deliver superior signal-to-noise ratios and exhibit comparable sensitivity to enhanced chemiluminescence (ECL)-based methods in western blot applications. No stripping and re-probing or multiple blots are required for multiple protein detection as commonly needed with ECL or colorimetric detection. Differently labeled WesternDot antibodies can be applied to a single blot for simultaneous detection of multiple proteins using standard gel or blot imaging platforms (a list of instruments that are compatible with WesternDot detection can be found in the user manual below). Our WesternDot antibodies utilize Qdot nanocrystals enhanced with VIVID technology, making them brighter than the original Qdot reagents. Additionally, they have reduced intensity differences between the different colors, simplifying multiplex applications.靶标信息Obtain superior images with the new Invitrogen™ Alexa Fluor™ Plus secondary antibodies. Make your low abundant targets visible, minimize time spent optimizing, and make your precious samples count. Alexa Fluor™ Plus secondary antibodies provide brighter signal while having lower cross-reactivity compared to leading Alexa Fluor secondary antibodies, providing you with higher sensitivity and better signal-to-noise for your critical experiments. Alexa Fluor Plus secondary antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor Plus secondary antibodies are conjugated using new, proprietary dye chemistry and are pre-adsorbed to reduce background and cross-reactivity so you can generate stunning images. Alexa Fluor Plus secondary antibodies provide up to 4.2 fold higher signal-to-background in imaging applications and up to 5.8 fold higher signal-to-background in fluorescent western applications compared to Alexa Fluor secondary conjugates.

数据

Mouse IgG (H+L) Secondary Antibody (W10805) in WBSimultaneous detection of three proteins on a single blot using Qdot® secondary antibody conjugates. A western blot containing serial dilutions (20-3 µg protein) of lysates from unstimulated (lanes 2-5) and hEGF-stimulated (lanes 6-9) A431 cells was probed with mouse anti-EGFR, rabbit anti-phospho-EGFR, and chicken anti-GAPDH antibodies, followed by (A) WesternDot® 800 goat anti-mouse (pseudocolored blue), (B) WesternDot® 605 goat anti-rabbit (pseudocolored red), and (C) WesternDot® 655 goat anti-chicken (pseudocolored green) conjugates. (D) The merged image shows overlaid red and blue bands as purple. The blot contains MagicMark™ XP Western Protein Standard (lane 1,Product # LC5603) and was imaged using the Fujifilm® LAS-4000 gel imager. Mouse IgG (H+L) Secondary Antibody (W10805)WesternDot® secondary antibodies can be used in existing Western Blot protocols. Fluorescent western detection is more convenient than chemiluminescence (ECL) methods. Blots are ready to image immediately after the secondary antibody application. There are no additional substrates or enzymatic/timing concerns, and the fluorescent signals are stable indefinitely.

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