F(ab')2-Goat anti-Mouse IgG (H+L) Secondary Antibody, Qdot 585/F(ab')2-山羊抗小鼠IgG (H+L)二抗Qdot 585

货号：Q-11011MP

规格：200 µL

价格：6812

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Gamma Immunoglobins Heavy and Light chains

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：其它

抗体类型:	二抗	同型对照：
浓度：		用法：	1:50（Flow）； 1:50 (ICC)；1:50 (IF); 1:50 (IHC); 1:50 (WB)

产品详细信息Qdot nanocrystals are composed of semi-conductor material to generate a fluorescent particle which is exceptionally bright and does not photobleach. Qdot nanocrystals paired with the correct optical filters are as much as 50 times brighter than traditional organic dyes.靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG (H+L) Secondary Antibody (Q-11011MP) in IFImmunofluorescence analysis of F(ab')2-Goat anti-Mouse IgG (H+L) Secondary Antibody, Qdot® 585 conjugate was performed using HeLa cells stained with alpha Tubulin (236-10501) Mouse Monoclonal Antibody (Product # A11126). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2 µg/mL primary antibody for 3 hours at room temperature. F(ab')2-Goat anti-Mouse IgG (H+L) Secondary Antibody, Qdot® 585 conjugate (Product # Q-11011MP) was used at a dilution of 1:50 in phosphate buffered saline containing 0.2% BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: red). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Alexa Fluor® 488 Phalloidin (Product # A12379), 1:300) (Panel c: green). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification. Mouse IgG (H+L) Secondary Antibody (Q-11011MP) in IF BPAE cells were plated on coverslips overnight. The next day the cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (Product # R37602) according to protocol. Cells were then incubated with 3 µg/mL anti-ATP Synthase Subunit IF1 antibody (Product # A-21355) for labeling of mitochondria for 60 min at room temperature followed by three washes with dPBS. Cells were then incubated with a 1:1000 dilution of goat anti-mouse Qdot® 655-conjugated secondary antibody (Product # Q-11011MP) for 60 min, followed by three washes with dPBS. Cells were labeled with NucBlue® Live cell stain (Product # R37605) and ActinGreen™ 488 ReadyProbes® reagent (Product # R37112) according to protocol. Coverslips were then mounted using ProLong® Gold Antifade Reagent (Product # P36930). Images were taken using the EVOS® FL Auto Imaging System and a 40X Coverslip corrected objective (Product # AMEP4699). Mouse IgG (H+L) Secondary Antibody (Q-11011MP) in IF BPAE cells were plated on coverslips overnight. The next day the cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (Product # R37602) according to protocol. Cells were then incubated with 3 µg/mL anti-ATP Synthase Subunit IF1 antibody (Product # A-21355) for labeling of mitochondria for 60 min at room temperature followed by three washes with dPBS. Cells were then incubated with a 1:1000 dilution of goat anti-mouse Qdot® 655-conjugated secondary antibody (Product # Q-11011MP) for 60 min, followed by three washes with dPBS. Cells were labeled with NucBlue® Live cell stain (Product # R37605) and ActinGreen™ 488 ReadyProbes® reagent (Product # R37112) according to protocol. Coverslips were then mounted using ProLong® Gold Antifade Reagent (Product # P36930). Images were taken using the EVOS® FL Auto Imaging System and a 40X Coverslip corrected objective (Product # AMEP4699).

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

Journal of Cancer Capture and Identification of Heterogeneous Circulating Tumor Cells Using Transparent Nanomaterials and Quantum Dots-Based Multiplexed Imaging.

技术参数

产品应用 Flow;ICC;IHC;IF;WB

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-21715.htm