Rabbit anti-Mouse IgG, IgM (H+L) Secondary Antibody, HRP/兔抗小鼠IgG，IgM（H + L）二抗，HRP

货号：31457

规格：1.5 mL

价格：4050

产品类型：荧光二抗

品牌：Thermo Fisher

物种：小鼠

宿主：兔

抗体亚型：IgG

荧光染料：HRP

类型:	二抗	同型对照：
浓度：	0.8 mg/mL	用法：	1:500-1:5,000(ICC)；1:500-1:5,000(IHC)；1:10,000-1:200,000(WB)

产品详细信息Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.This antibody has been successfully used in Western blot, and ICC applications.Antibody Specificity: When tested by immunoelectrophoresis, this antibody reacts with the heavy chains of mouse IgG and IgM and with light chains common to most mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.Restoration and Storage: Store product at 4°C until opened. Restore with 1.5 mL distilled water (0.8 mg/mL after restoration). Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. To judge clarity, draw product into a pasteur pipette. Product may be stored for several weeks at 4°C as an undiluted liquid. After dilution, do not use for more than one day.To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.Country of Origin: USA靶标信息IgM (Immunoglobulin M) is expressed intracellularly during the early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. The isotype of a primary antibody and its application can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are also useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

数据

Mouse IgG, IgM (H+L) Secondary Antibody (31457) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of U-87 MG (Lane 1) and K-562 (Lane 2). The blots were probed with Anti-SOD2 Mouse Monoclonal Antibody (Product # MA1-106, 2 µg/mL) and detected by chemiluminescence using Rabbit anti-Mouse IgG, IgM (H+L) Secondary Antibody, HRP conjugate (Product # 31457) at dilutions 1:5,000 (Fig. 1), 1:10,000 (Fig. 2) and 1:20,000 (Fig. 3). A 22 kDa band corresponding to SOD2 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE®12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106)

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参考文献：

1.Reproductive biomedicine online Replacement of sodium with choline in slow-cooling media improves human ovarian tissue cryopreservation.

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