F(ab')2-Goat anti-Mouse IgG, IgM (H+L) Secondary Antibody, Alexa Fluor 488/F(ab')2-山羊抗小鼠IgG, IgM (H+L)二抗，Alexa Fluor 488

货号：A-10684

规格：500 µg

价格：3549

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Mouse IgG/IgM (H+L)

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：Alexa Fluor 488

抗体类型:	荧光二抗	同型对照：
浓度：	2 mg/mL	用法：	0.1 µg/mL (ICC); 0.1 µg/mL (IF)

点击查看所有Alexa Fluor 荧光二抗靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG, IgM (H+L) Secondary Antibody (A-10684) in IF Immunofluorescence analysis of F (ab')2-Goat anti-Mouse IgG/IgM (H+L) Secondary Antibody, Alexa Fluor 488 (Product # A-10684) was performed using MCF-7 cells stained with Cytokeratin 19 Mouse Monoclonal Antibody (Product # MA5-12613). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with mouse primary antibody (1:250 dilution) for 3 hours at room temperature. F (ab')2-Goat anti-Mouse IgG/IgM (H+L) Secondary Antibody, Alexa Fluor 488 was used at concentration of 0.1 µg/mL in phosphate buffered saline containing 0.2 % BSA for 45 minutes at room temperature, for detection of cytokeratin 19 in the membrane (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Rhodamine Phalloidin (Product # R415, 1:300) (Panel c: red). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification.

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参考文献：

1. Biomedical reportsInfluence of high-altitude hypoxic environments on the survival of cochlear hair cells and spiral ganglion neurons in rats.2. Nature neuroscienceHedgehog signaling promotes basal progenitor expansion and the growth and folding of the neocortex.3. Journal of visualized experiments : JoVEIsolation of Sertoli Cells and Peritubular Cells from Rat Testes.4.Biomedical optics expressSimple buffers for 3D STORM microscopy.5.Pigment cell and melanoma researchProteasome inhibition and ROS generation by 4-nerolidylcatechol induces melanoma cell death.

技术参数

产品应用 ICC;IF;IHC(F);Flow

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