F(ab')2-Goat anti-Mouse IgG (H+L) Secondary Antibody, Functional Grade, eBioscience/F（ab'）2-山羊抗小鼠IgG（H + L）二抗，Functional Grade

货号：16-5098-85

规格：500 µg

价格：3274

产品类型：荧光二抗

品牌：Thermo Fisher

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：其它

类型:	二抗	同型对照：
浓度：	1 mg/mL	用法：	Assay-Dependent(Flow)；Assay-Dependent(FN)

产品详细信息Description: This polyclonal F(ab')2 fragment recognizes mouse IgG. Reactivity to human, bovine, and horse has been removed. This polyclonal antibody can be used to crosslink and activate B lymphocytes.Applications Reported: This polyclonal F(ab')2 Goat Anti-Mouse IgG has been reported for use in functional assays (such as B cell activation). Concentrations should be determined empirically for optimal performance in the assay of interest.Applications Tested: F(ab')2 Anti-Mouse IgG Functional Grade Purified has been tested by in vitro stimulation of mouse splenocytes.Endotoxin: Less than 0.1 ng/µg antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondaryantibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG (H+L) Secondary Antibody (16-5098-85) in FlowMouse splenocytes were unstimulated (left), stimulated with 10 µg/mL of F (ab')2 Anti-Mouse IgG Functional Grade Purified (middle), or stimulated with 10 µg/mL of F (ab')2 Anti-Mouse IgG Functional Grade Purified plus 5 µg/mL of Anti-Mouse CD40 Functional Grade Purified (Product # 16-0401-82) (right) for 24 hours.Cells were surface stained with Anti-Mouse CD19 APC (Product # 17-0193-82) and Anti-Mouse CD69 FITC (Product # 11-0691-82).Cells in the lymphocyte gate were used for analysis.

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