Rat anti-Mouse IgG1 Secondary Antibody, PE-Cyanine7, eBioscience™

货号：25-4015-82,25-4015-80

规格：100 μg,25μg

价格：1855,0

产品类型：荧光二抗

品牌：Thermo Fisher

物种：小鼠

宿主：大鼠

抗体亚型：IgG

克隆号：M1-14D12

荧光染料：PE-Cy7

抗体类型:	荧光二抗	同型对照：
浓度：	0.2 mg/mL	用法：	0.5 µg/test (Flow)

产品详细信息Description: The monoclonal antibody M1-14D12 recognizes Mouse IgG1 antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG1 or any rat isotype antibodies.Applications Reported: This M1-14D12 antibody has been reported for use in flow cytometric analysis.Applications Tested: This M1-14D12 antibody has been tested by flow cytometric analysis of cells stained with a mouse IgG1 primary antibody. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered. 靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据：

Mouse IgG1 Secondary Antibody (25-4015-82) in FlowStaining of normal human peripheral blood cells with Anti-Human CD20 FITC (Product # 11-0209-42) and Anti-Human CD4 Purified (a mouse IgG1 isotype) (Product # 14-0049-82) followed by staining with 0.25 µg of Rat IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4301-82) (left) or 0.25 µg of Anti-Mouse IgG1 PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1.mBioMultiple Histone Lysine Methyltransferases Are Required for the Establishment and Maintenance of HIV-1 Latency.2.PloS oneVirulence-Dependent Alterations in the Kinetics of Immune Cells during Pulmonary Infection by Mycobacterium tuberculosis.3.PloS oneCD36 contributes to malaria parasite-induced pro-inflammatory cytokine production and NK and T cell activation by dendritic cells.4.GastroenterologyExpression of intercellular adhesion molecule 1 by hepatocellular carcinoma stem cells and circulating tumor cells.

技术参数

产品应用 Flow

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-21788.htm